Identification of MYB Transcription Factors Family and Screening MYB Genes Associated with Pollen Abortion in Lilium Asiatic hybrids
WANG Xue-Qian1, 2, YUAN Guo-Zhen1, 2, WU Ze1, 2, LI Qian1, 2, TENG Nian-Jun1, 2, *
1 College of Horticulture, Nanjing Agricultural University/Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China;
2 Baguazhou Modern Innovative Technology Center of Nanjing Agriculture University, Nanjing 210043, China
Abstract：MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family play an important role in regulating plant reproduction and growth, LaMYB transcription factors in Liliium Asiatic hybrids 'Little Kiss' were identified at the transcriptome level and applied bioinformation analysis which aim to screen out those genes regulating pollen abortion. Based on the transcriptome data of lily anther, the MYB protein sequences were identified by Pfam 31.0 and BLASTP, at the same time, conserved domain and phylogenetic tree analysis were constructed by using Web Logo 3.0 and MEGA 7.0 for those R2R3-MYB transcription factors with the largest proportion. The expression levels of meiosis stage and mononuclear stage of LaMYB were analyzed by Mev. The results showed that 69 MYB genes were identified in lily, 50 of them belonged to R2R3-MYB, 15 of them belonged to 1R-MYB and 4 of them belonged to 3R-MYB, in the mean time, all those LaMYB proteins were unstably hydrophilic proteins; subcellular localization prediction showed that LaMYB proteins were all localized in nucleus; Web logo3.0 analysis found that the protein domain of R2R3-MYB were relatively conserved, but the variability of its C-terminal transcriptional activation domain makes it functionally diverse. The phylogenetic of LaMYB proteins were divided into 22 subfamilies according to the classification method of Arabidopsis family. The homologous genes involved in the division of pollen abortion mainly belong to the S18、S23、S21 and S22 subfamilies. Meanwhile, the results of RNA-Seq analysis indicated that the expression level of 42 LaMYB genes were up-regulated in the key period of pollen abortion (mononuclear stage) compared with the normal developmental stage (meiosis stage)), the other 27 genes were down-regulated in all of LaMYB. Additionally, the result of qRT-PCR showed that the 6 candidate genes were significantly different between the normal developmental stage and abortion period of lily pollen. This study showed that LaMYB genes play an important regulatory role in pollen abortive process and providing basic information for further exploration of the function of LaMYB genes in pollen abortion.
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