Cloning of NAC Transcription Factor Gene GhSNAC1 and Its Drought and Salt Resistance in Upland Cotton (Gossypium hirsutum)
WANG Li-Guo, FU Ming-Chuan, LI Hao, LIU Ren-Zhong, LIU Zhan-Ji*
Key Laboratory of Cotton Breeding and Cultivation in Huang-Huai-Hai Plain, Ministry of Agriculture/Shandong Cotton Research Center, Jinan 250100, China
Abstract：NAC (NAM/ATAF/CUC) family is a class of transcription factors unique to plants, and plays crucial roles on developmental regulation, hormone signal transduction, biotic and abiotic stress responses. In the present study, a stress-responsive NAC gene, named as GhSNAC1 (GenBank No. KU759894), was isolated from upland cotton (Gossypium hirsutum) via reverse transcription PCR (RT-PCR). The sequence analysis indicated that GhSNAC1 was 1 104 bp in length and encoded a protein of 299 amino acids with a relative molecular mass of 33.9 kD and an isoelectric point of 6.18. GhSNAC1 contained a conserved NAC domain in the N-terminus, indicating that it was a typical NAC transcription factor. The phylogenetic analysis indicated that GhSNAC1 had extremely high similarity with ATAF1 (Arabidopsis transcription activation factor 1) from Arabidopsis thaliana. qRT-PCR anaylsis showed that GhSNAC1 was induced by treatments of drought, salt, cold and Verticillium dahliae which suggested that GhSNAC1 might be involved in responses to biotic and abiotic stresses. GhSNAC1 was then overexpressed in transgenic tobacco (Nicotiana tabacum) plants. Transgenic lines and wild type (WT) were used to evaluate the germination and seedling growth under either salt (100 mmol/L NaCl) or drought (200 mmol/L mannitol) stress. The results indicated that drought and salt stresses significantly inhibited the germination of WT seeds, and the germination rate of WT was below 50%, while the germination of transgenic lines was above 80%. The WT seedlings were small and weak. However, the transgenic lines were vigorous. The fresh weight per plant and root length of transgenic lines were more than twice and 1.5 times of those of wild type, respectively. Collectively, the above results suggested that overexpression of GhSNAC1 in tobacco could improve tolerance to drought and salt stresses. The present study laid a foundation for further exploring the molecular mechanism of drought and salt tolerance of GhSNAC1.
王立国, 傅明川, 李浩, 刘任重, 柳展基. 陆地棉NAC转录因子基因GhSNAC1的克隆及其抗旱耐盐分析[J]. 农业生物技术学报, 2019, 27(4): 571-580.
WANG Li-Guo, FU Ming-Chuan, LI Hao, LIU Ren-Zhong, LIU Zhan-Ji. Cloning of NAC Transcription Factor Gene GhSNAC1 and Its Drought and Salt Resistance in Upland Cotton (Gossypium hirsutum). 农业生物技术学报, 2019, 27(4): 571-580.
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