Cloning and Expression Analysis of CmPAN Gene in Chrysanthemum morifolium cv. Huaihuang
ZHAO Xi-Ting1, 2, 3, *, LIU Ke1, ZHU Yu-Ting1, MA Meng-Dan1, LV Meng1, SONG Ling-Yu1, JIANG Li-Wei1, WANG Miao1, LI Ming-Jun1, 2, 3
1 College of Life Science, Henan Normal University, Xinxiang 453007, China; 2 Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province, Xinxiang 453007, China; 3 Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Universities of Henan Province, Xinxiang 453007, China
Abstract：Chrysanthemum morifolium originated from the ancient Huaiqingfu is one of the Four Famous Huai Medicines, which has high ornamental, edible and medicinal value. But it is rare reported about the research on its flower organ development. To study the function of PERIANTHIA (PAN) gene in flower development of Ch. morifolium, a 1 484 bp full-length cDNA sequence of homologous PAN gene was cloned by employing homology gene cloning and Rapid amplification of cDNA ends (RACE) from Ch. morifolium cv. Huaihuang. The cDNA sequence contained 64 bp 5' UTR, 106 bp 3' UTR and 1 314 bp CDS which encoded 437 amino acids. The protein of CmPAN had very high similarity with PANs from other plant species. C-terminal amino acids sequence of CmPAN included group D structural features with a well characterized family of plant bZIPs (basic leucine zipper motif) and 2 glutamine-rich regions. CmPAN was most close to AtPAN, so the cloned sequence was named CmPAN (GenBank No. KX380854). Putative protein molecular weight was 48.265 kD, the theoretical isoelectric point was 8.82, and it was non-secretory protein with nuclear localization sequence, its disordered degree was smaller than that of AtPAN. The qRT-PCR detection showed that CmPAN was higher expressed in flowers and stems than that in roots and leaves, and the CmPAN expession in floral development increased from bud emergence stage to visible color stage, then decreased at late phase. The CmPAN expession in floral organ ranked from high to low was petal of tubular flower, stamen of tubular flower, petal of ray floret, carpel of ray floret and receptacle. As a result, CmPAN might have important roles in early phase of floral development, and be closely related to the development of petals and stamens of tubular flower. The study provides some reference for further exploring the function of CmPAN gene.
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