Cloning of ZlGH3-8 Gene and Its Expression Analysis in Zizania latifolia During Development
ZHAO Jin-Lan*, ZHOU Mei-Qi*, YE Zi-Hong, YAN Mu-Xi, ZHANG Ya-Fen, XIA Wen-Qiang, CUI Hai-Feng**
Zhejiang Provincial Key Laboratory of Biometrology and Inspection / Quarantine College of Life Sciences, China Jiliang University, Hangzhou 310018, China
Abstract：Pregnant Zizania latifolia is the result of stem organ expansion and development induced by Ustilago esculenta after infecting Zizania latifolia plants, but the regulatory mechanism of its expansion and development is still unclear. In this study, the full-length sequence of ZlGH3-8 (Zizania latifolia Gretchen Hagen 3-8) gene (GenBank No. MH355951) was cloned, the gene sequence was 1 916 bp in length and contained 2 introns. The cDNA was 1 174 bp in length, encoded 390 amino acids, which had a GH3 domain and a indoleacetic acid amide synthetase domain, and was highly similar to OsGH3-8 (GenBank No. XP_015647797.1) of Oryza sativa ssp. japonica. It was found that the expression change of ZlGH3-8 in plants was related to mycelial infection and proliferation of U. latifolia. The expression of ZlGH3-8 in stems was significantly higher than that in leaves (P<0.05). At the early stage of stem enlargement, the expression of ZlGH3-8 in gray Z. latifolia was significantly higher than that of white Z. latifolia, while the expression of male Z. latifolia was the lowest (P<0.05). Further analysis found that there were significant differences in the expression of ZlGH3-8 during the expansion and development of white Z. latifolia, indicating the ZlGH3-8 might be participated in the morphological development of stem organs of Z. latifolia, and it might be related to the down-regulation of immune defense response related to the infection of U. latifolia during pregnancy. This study preliminarily clarified the expression response of ZlGH3-8 in the development of Z. latifolia, which provides basic data for the study on the mechanism of expansion and development of Z. latifolia.
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