Abstract：We report here the isolation and characterization of microsatellites, in Pelteobagrus fulvidraco using an enrichment protocol. The size-fractionated genomic library was screened with (AC)8、(CT)8、(AT)7、(GATA)8 and (GATT)7 oligonucleotide probes. A total of 38 clones were identified as having the microsatellite repeats, and specific 22 primer pairs were designed for 38 microsatellite loci. Of the 22 primer pairs, 18 amplified polymorphic loci in Pelteobagrus fulvidraco . At these polymorphic loci, the number of alleles per locus varied from 2 to 8（the average 3.78）, the observed heterozygosities ranged from 0.2225 to 0.8101（the average 0.5755）, and the polymorphism information content ranged from 0.3425 to 0.7900（the average0.5336）. These 18 primer pairs were examined also in Pelteobagrus species and Siluriformes species.16 of the 18 primer pairs amplified the loci in the Pelteobagrus species,13 of the 18 primer pairs amplified the loci in the Siluriformes species. These results indicate that the microsatellites isolated in this study were highly informative and could be useful tools for genetic analysis in the Pelteobagrus species and the Siluriformes species.
