Association Between Polymorphism of MHC-DQB2 Gene Exon 2 and Susceptibility to Coccidia in Shanbei White Cashmere Goats (Capra hircus)
SONG Xiao-Yue, SHI Lei, LIU Jin-Wang, ZHU Hai-Jing, LI Long-Ping, QU Lei
1 Shaanxi Province Engineering & Technology Research Center of Shanbei Cashmere Goats/Yulin University, Yulin 719000, China; 2 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 3 Shaanxi Province Jia County Tong Town Animal Husbandry & Veterinary Station, Yulin 719200, China;
Abstract：Major histocompatibility complex gene (MHC) can encode antigen-presenting molecule to initiate immune response of the body, which has great potential in animal disease-resistance breeding. In this study, the correlation between MHC-DQB2 gene and Coccidia infection in Shanbei white cashmere goats (Capra hircus) was analyzed to provide basic data for the mining and application of disease-resistant genes in goats. MHC-DQB2 gene exon 2 was analyzed by bioinformatics and population genetics in 104 Shanbei white cashmere goats, infection of Coccidia was detected and the correlation between DQB2 and coccidiosis was analysed. A total of 15 alleles and 27 genotypes were obtained. The results showed that DQB2 had high polymorphism and under positive selection pressure in the course of evolution, the frequency of each allele in the population had significant difference. In this study, the infection rate of Coccidia was 71.43%, and moderate intensity infection (1×105≥oocyst per gram (OPG) ≥1×104) was predominant. Correlation analysis revealed that DQB2*03 allele and 0311 genotype were negatively correlated with Coccidia infection (P＜0.05), indicating that DQB2*03 allele and 0311 genotype might probably be the susceptibility allele or genotype of coccidiosis. The results of this study will help to clarify the role of MHC molecular markers in the immunity of Shanbei white cashmere goats, provide experimental data support for the correlation between MHC genes and diseases, and promote the application in disease-resistance breeding.
宋晓越, 史雷, 刘锦旺, 朱海鲸, 李陇平, 屈雷. 陕北白绒山羊MHC-DQB2基因外显子2多态性与球虫易感性的相关分析[J]. 农业生物技术学报, 2019, 27(9): 1629-1636.
SONG Xiao-Yue, SHI Lei, LIU Jin-Wang, ZHU Hai-Jing, LI Long-Ping, QU Lei. Association Between Polymorphism of MHC-DQB2 Gene Exon 2 and Susceptibility to Coccidia in Shanbei White Cashmere Goats (Capra hircus). 农业生物技术学报, 2019, 27(9): 1629-1636.
1 李微, 韩彩霞, 畅丹, 等. 2009. 黑龙江省哈尔滨地区鸡球虫的区系调查[J]. 中国兽医科学, 39(09): 821-824. (Li W, Han C X, Chang D, et al.2009. Investigation of Coccidia fauna in chickens in Harbin prefecture of Heilongjiang province[J]. Chinese Veterinary Science, 39(09): 821-824.) 2 宋晓育, 张小丽, 马小军, 等. 2015. 甘肃高山细毛羊和小尾寒羊DQB1基因第2外显子多态性及其与乳房炎相关性[J]. 江苏农业学报, 31(5): 1070-1077. (Song X Y, Zhang X L, Ma X J, et al.2015. Polymorphism of ovine major histocompatibility complex classⅡ DQB1 gene exon 2 and its correlation with mastitis[J]. Jiangsu Journal of Agricultural Sciences, 31(5): 1070-1077.) 3 杨玉荣, 李艳玲, 陈二平, 等. 2018. 家兔球虫病的感染流行调查及防治措施[J]. 湖北农业科学, 57(6): 98-99, 126. (Yang Y R,Li Y L, Chen E P, et al.2018. Rabbit coccidiosis infection epidemic investigation and prevention and control measures[J]. Hubei Agricultural Sciences, 57(6): 98-99, 126.) 4 Ali A O, Stear A, Fairlie-Clarke K, et al.2017. The genetic architecture of the MHC classⅡregion in British Texel sheep[J]. Immunogenetics, 69(3): 157-163. 5 Aust S, Felix S, Auer K, et al.2017. Absence of PD-L1 on tumor cells is associated with reduced MHCⅠexpression and PD-L1 expression increases in recurrent serous ovarian cancer[J]. Scientific Reports, 7: 42929. 6 Ballingall K T, Lantier I, Todd H, et al.2018. Structural and functional diversity arising from intra- and inter-haplotype combinations of duplicated DQA and B loci within the ovine MHC[J]. Immunogenetics, 70(4): 257-269. 7 Corley M M, Savage A.2015. Expression of the DRB1*1101 allele in meat goats pasture exposed to Haemonchus contortus[J]. Journal of Agricultural Science, 7(11): 19-27. 8 Gelasakis A I, Arsenos G, Hickford J.et al.2013. Polymorphism of the MHC-DQA2 gene in the Chios dairy sheep population and its association with footrot[J]. Livestock Science, 153(1-3): 56-59. 9 Guo X F, Wang X Y, Di R, et al.2018. Metabolic effects of FecB gene on follicular fluid and ovarian vein serum in sheep (Ovis aries)[J]. International Journal of Molecular Sciences, 19(2): 539-552. 10 He X H, Song S, Chen X F, et al.2018. Genome-wide association analysis reveals the common genetic locus for both the typical and atypical polycerate phenotype in Tibetan sheep[J]. Animal Genetics, 49(2): 142-143. 11 Larruskain A, Minguijon E, Garcia-Etxebarria K, et al.2010. MHC classⅡ DRB1 gene polymorphism in the pathogenesis of Maedi-Visna and pulmonary adenocarcinoma viral diseases in sheep[J]. Immunogenetics, 62(2): 75-83. 12 Li R Y, Hui W Q, Jia B, et al.2011. The relationship between MHC-DRB1 gene second exon polymorphism and hydatidosis resistance of Chinese merino (Sinkiang Junken type), Kazakh and Duolang sheep[J]. Parasite, 18(2): 163-169.
