黄精内生菌枯草芽胞杆菌HJ-2的抑菌活性研究

doi:10.3969/j.issn.1674-7968.2019.09.015

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摘要植物内生菌可产生与宿主植物相同或相似的代谢产物,是筛选结构新颖次级代谢产物的重要资源;因此从药用植物黄精(Polygonatum cyrtonema)中分离筛选内生菌、研究其代谢产物抑菌活性具有重要意义。黄精内生菌HJ-2 (GenBank No. MK774703)是分离自野生黄精根状茎并经初筛和鉴定的一株枯草芽胞杆菌(Bacillus subtilis)。为综合开发与利用该菌株的代谢产物,本研究采用多种培养基对菌株HJ-2进行发酵,并分别用甲醇、乙醇、正丁醇、丙酮和乙酸乙酯对菌体及其发酵液的活性物质进行萃取,探究萃取产物的理化性质及抑菌活性。结果表明,菌株HJ-2产生的抑菌物质主要存在于发酵液中,且对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)和绿脓杆菌(Pseudomonas aeruginosa)均具有显著抑菌效果。对发酵产物进行耐酸碱性和耐热性实验,结果表明该产物能够耐65 ˚C高温,但对酸碱耐受能力稍差。本研究为发掘安徽地区的黄精内生菌资源和综合开发利用菌株HJ-2提供了理论基础。

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	翟大才
	房震
	吕彩云
	张明亮
	刘珊
	吴梦
	方茹月
	周讯
	方建新
	柏晓辉

关键词 ：黄精, 内生菌, 发酵产物, 抑菌活性, 稳定性

Abstract：Endophytes, which can produce the same or similar metabolites as host plants, is an important resource for screening novel secondary metabolites. Thus, it has great significance to isolate and screen antagonistic endophytic bacterium from the wild Polygonatum cyrtonema and study the antibacterial activity of its metabolites. The endophytic bacterium HJ-2 (GenBank No. MK774703) isolated and screened from the rhizome of the wild P. cyrtonema was identified as Bacillus subtilis. In order to furtherly utilize the strain, different media were used for fermentation of the strain. The active substances were extracted from the bacterial cell of strain HJ-2 and its fermentation broth by methanol, ethanol, n-butanol, acetone and ethyl acetate, respectively. All the extracted products were used for physicochemical properties and antibacterial activity study. Our results showed that the antibacterial active substances produced by the strain were presented in the fermentation broth and had significant antibacterial effects against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The fermentation products were further tested for acid and alkali resistance and heat resistance. The results showed that the products could resistance to high temperature of 65 ˚C, but had a poor performance of acid and alkali resistance. This study provides a theoretical basis for the exploration of endophytic bacteria resources of P. cyrtonema from Anhui province and the comprehensive development and utilization of strain HJ-2.

Key words： Polygonatum cyrtonema Endophytes Metabolites Antibacterial activity Stability

收稿日期: 2019-02-10

ZTFLH:

S182

基金资助:国家林业公益性行业科研专项(No. 201304407)、国家林木种质资源共享服务平台(No. 2005DKA21003)、安徽省林业科技创新研究项目(No. AHLYCX-2018-10和No. AHLYCX-2018-29)、安徽省创新型省份资金(No. 2018XZX002)、黄山学院自然科学研究项目(No. 2016xkjq005)、安徽省自然科学研究项目(No. 1908085QC124)、安徽省教育厅质量工程项目(No. 2015sjjd022)和国家大学生创新创业项目(No. 201810375032和No.201810375049)

通讯作者: bxh@hsu.edu.cn

引用本文:

翟大才, 房震, 吕彩云, 张明亮, 刘珊, 吴梦, 方茹月, 周讯, 方建新, 柏晓辉. 黄精内生菌枯草芽胞杆菌HJ-2的抑菌活性研究[J]. 农业生物技术学报, 2019, 27(9): 1664-1672.
ZHAI Da-Cai, FANG Zhen, LV Cai-Yun, ZHANG Ming-Liang, LIU Shan, WU Meng, FANG Ru-Yue, ZHOU Xun, FANG Jian-Xin, BAI Xiao-Hui. Research on Antibacterial Activity of the Endophytic Bacillus subtilis HJ-2 from Polygonatum cyrtonema. 农业生物技术学报, 2019, 27(9): 1664-1672.

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http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.09.015 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I9/1664

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