Genetic Analysis and Gene Mapping of Leaf Number of Flue-cured Tobacco (Nicotiana tabacum) Variety 'Jiucaiping 2'
LIANG Ting1, QUE Yuan-Hui2, YU Qi-Wei3, JIA Rong-Li2, ZHOU Guang2, GUO Kai-Yang2, HUANG Ying2, LIU Ren-Xiang2,*
1 College of Agriculture, Guizhou University/Key Laboratory of Tobacco Quality Research of Guizhou Province, Guiyang 550025, China;
2 College of Tobacco, Guizhou University/Key Laboratory of Tobacco Quality Research of Guizhou Province, Guiyang 550025, China;
3 Bijie City Company of Guizhou Tobacco Company, Bijie 551700, China
Abstract：Leaf number is one of the important factors affecting the yield of flue-cured tobacco (Nicotiana tabacum). To improve the efficiency of leaf number genetic improvement, this studying took the flue-cured tobacco variety 'NC82' with few leaves and the flue-cured tobacco variety 'Jiucaiping 2' with many leaves as the parents, constructed F1 and F2 genetic populations, and conducted genetic analysis on the leaf number of 'Jiucaiping 2'. Bulked segregant analysis coupled with whole-genome sequencing (BSA-seq) and kompetitive allele-specific PCR (KASP) were used to map the leaf numbers of 'Jiucaiping 2'. The results showed that the number of leaves of the flue-cured tobacco variety 'Jiucaiping 2' was a quantitative trait mainly controlled by additive effect genes. At the same time, it was regulated by genes with partial recessive relationships, and the number of leaves was partially dominant. BSA-seq technology was used for initial mapping, combined with the Δ(SNP-index) locus and euclidean distance (ED) locus association analysis obtained by sequencing, the candidate range of leaf number was located in the 6.96 Mb range of chromosome 9. According to the SNP genotypes obtained by resequencing, KASP markers were developed in the candidate region to detect F2 individual genotypes, and the candidate gene of leaf number was further reduced to the 1.92 Mb region, which contained 37 genes. Sequence analysis and functional prediction of genes within this region indicated 8 key genes controlling leaf number traits of 'Jiucaiping 2'. This study provides basic data for further clarifying key genes regulating leaf number of flue-cured tobacco in 'Jiucaiping 2', improving yield breeding efficiency of flue-cured tobacco, and revealing the molecular mechanism of leaf number formation of flue-cured tobacco.
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