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2025年4月4日 星期五
农业生物技术学报  2023, Vol. 31 Issue (1): 36-49    DOI: 10.3969/j.issn.1674-7968.2023.01.004
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
番茄DIR基因家族的全基因组鉴定及其表达分析
孙会茹1,2,*, 张嘉宁1, 任敏1, 王延峰1,2,*
1 延安大学 生命科学学院,延安 716000;
2 延安大学 陕西省红枣重点实验室,延安 716000
Genome-wide Identification and Expression Analysis of DIR Gene Family in Tomato (Solanum lycopersicum)
SUN Hui-Ru1,2,*, ZHANG Jia-Ning1, REN Min1, WANG Yan-Feng1,2,*
1 College of Life Sciences, Yan'an University, Yan'an 716000, China;
2 Shaanxi Key Laboratory of Chinese Jujube, Yan'an University, Yan'an 716000, China
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摘要 DIR (dirigent)作为植物木质素和木脂素生物合成过程中的一类重要调控蛋白,在植物生长发育和胁迫应答等过程中发挥重要作用。为了探究番茄(Solanum lycopersicum) DIR家族基因的生物学功能,本研究对SlDIR基因家族进行了生物信息学分析,利用TFGD网站公布的转录组数据和qRCR技术,检测SlDIR家族基因在不同组织、盐和干旱胁迫及脱落酸(abscisic acid, ABA)处理下的表达特点。结果显示:番茄中共有31个SlDIR基因,不均匀分布在除Chr03外的11条染色体上,包含3个串联重复基因簇,且多数SlDIR基因无内含子。系统进化分析显示,番茄DIR基因家族成员可分为3个亚族,各亚族基因数量不等。所有SlDIR蛋白均具有dirigent结构域,多数成员还具有信号肽;同一亚族的SlDIR蛋白保守基序分布和排列相似。此外,植物激素和胁迫响应元件在SlDIR家族基因启动子区域分布广泛。分析丁香假单胞杆菌番茄致病变种无毒菌株(Pseudomonas syringae pv. tomato DC3000, Pst DC3000)处理下SlDIR基因在不同组织和不同抗性材料的表达特征,发现多数SlDIR基因在根中优势表达,同时SlDIR5SlDIR9、SlDIR13SlDIR15SlDIR27的表达在番茄果实发育早期显著高于破色后。在Pst DC3000处理下,11个SlDIR基因的表达在抗性型和敏感型番茄品种间存在显著差异。ABA处理12 h时,7个SlDIR基因的表达显著上调;盐胁迫下,SlDIR27的表达受到显著诱导;干旱胁迫下,SlDIR13SlDIR27显著上调表达。研究结果为进一步研究SlDIR基因的生物学功能提供参考依据。
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孙会茹
张嘉宁
任敏
王延峰
关键词 番茄DIR基因家族生物信息学分析胁迫ABA表达分析    
Abstract:Dirigent genes (DIRs) are important regulator in the biosynthesis of plant lignin and lignans, and play pivotal roles in plant growth and development, and stress response. In order to explore the function of SlDIR family genes in tomato (Solanum lycopersicum), bioinformatics analysis of SlDIR members were carried out and expression characteristics of them in different organs, various stresses and abscisic acid (ABA) treatments were detected by released RNA-seq in TFGD website and qPCR. The results showed that 31 SlDIR genes were identified in tomato, which were unevenly distributed on 11 chromosomes (except Chr03) with 3 tandem duplication clusters. Most of SlDIR genes had no intron. The phylogenetic analysis displayed that the SlDIR family mumbers were classified into 3 subfamilies, the member numbers of which were various. All of SlDIR proteins had dirigent domain, and most of them also contained signal peptide. The distribution and arrangement of the motifs in SlDIRs from the same subfamily were similar. In addition, plant hormone and stress response elements were widely distributed in the promoter regions of SlDIRs. The expression characteristics of SlDIRs in different organs and different resistant materials under Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) treatment were further analyzed. It was found that most SlDIRs were predominance expressed in roots, and the expressions of SlDIR5, SlDIR9, SlDIR13, SlDIR15 and SlDIR27 were significantly higher in the early stages than that in the later stages of tomato fruit development. The significantly different expressions of 11 SlDIRs were displayed between resistant and sensitive tomato varieties under Pst DC3000 treatment. Under ABA treatment for 12 h, the expressions of 7 SlDIRs were significantly up-regulated. Salt stress significantly induced the expression of SlDIR27. Meanwhile, SlDIR13 and SlDIR27 showed up-regulated expressions significantly under drought treatment. The results of this study provide a reference for further study on the biological functions of SlDIR family genes.
Key wordsSolanum lycopersicum    DIR gene family    Bioinformatics analysis    Stress    ABA    Expression analysis
收稿日期: 2022-03-23     
ZTFLH:  S641.2  
基金资助:陕西省教育厅科研计划项目(21JK0993); 陕西省自然科学基础研究计划项目(2022JQ-159); 延安大学博士科研启动项目(YDBK2019-42)
通讯作者: *shrpiaoliu@163.com;yadxwyf@yau.edu.cn   
引用本文:   
孙会茹, 张嘉宁, 任敏, 王延峰. 番茄DIR基因家族的全基因组鉴定及其表达分析[J]. 农业生物技术学报, 2023, 31(1): 36-49.
SUN Hui-Ru, ZHANG Jia-Ning, REN Min, WANG Yan-Feng. Genome-wide Identification and Expression Analysis of DIR Gene Family in Tomato (Solanum lycopersicum). 农业生物技术学报, 2023, 31(1): 36-49.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2023.01.004     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2023/V31/I1/36
 
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