Abstract：Resistant starch has important physiological functions which reduce blood sugar levels of people with diabetes, reduce bowel dysfunction and colon cancer incidence of a disease, provide energy and prevent fat accumulation. In order to understand the genetic characteristics of resistant starch content in rice(Oryza sativa L.), the content of resistant starch of reciprocal cross F1 and F2, F2∶3 was analyzed derived from high resistant starch content variety Gongmi 3(indica) and low resistant starch content variety Nipponbare(japonica). The results indicated that the content of resistant starch and the morphology of starch granule of reciprocal cross F1 were similar to their female parent, but their F2 had no significant difference in resistant starch content, the characteristics separation in F2∶3. So the content of resistant starch was significant by the influence of maternal effect. Three pairs of SSR markers from chromosome 1 and 6 pairs of SSR markers from chromosome 6 were screened possible linkage with resistant starch loci by bulked segregation analysis of F2∶3 population of Gongmi3/Nipponbare. Three QTL loci(qRS6-1, sqRS6-2 and qRS6-3) were detected by QTL software. Among them, 20.3556% and 40.1349% phenotypic variation could be explained for the qRS6-2 and qRS6-3 of the total variation, respectively. Meanwhile, 1 epistasis effect was found between chromosome 1 and chromosome 6. The results demonstrated that resistant starch content was a quantitative trait controlled by a few main genes and several minor genes, the interactions of non-allelic genes. The above results provide theoretical basis for female parent choose of high resistant starch in rice breeding and make a solid foundation for fine mapping of main effect genes which associated with resistant starch content.
