Abstract：
Abstract: Potato virus Y(PVY) and Potato virus X(PVX) are two of the important viruses infecting potatoes. They often simultaneously affect potatoes, resulting in 60-80% reduction. Complex RT-PCR methods were established to detect the two viruses in this paper. The result was as follow: If Oligod(T)18 was as primer in reverse transcription, PVX couldn't be detected. The reason was that the differential character of cDNA was low. To improve that of cDNA, PVX and PVY's 3' primers participated in reverse transcription not Oligod(T)18, PVX and PVY were all detected. The conclusion was that in the multiply RT-PCR, 3' primers was used in the reverse transcription, the two viruses could be all detected.
