Expression Analysis of Three Stilbene Synthase Genes from Chinese Wild Vitis quinquangularis
WU Feng-Ying, LIU Meng-Qi, WANG Yue-Jin*
College of Horticulture/State Key Laboratory of Crop Stress Biology in Arid Areas/Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling 712100, China
Abstract：As a phytoalexin produced by plants under biotic and abiotic stresses, resveratrol is a metabolite of stilbene synthases (STSs). Chinese wild Vitis quinquangularis accession 'Danfeng-2' contains a high concentration of resveratrol in its fruits, which also possesses strong plant resistance against diseases. The expressions and functions study of its STS genes can serve as the disease-resistant germplasm resources for resistance breeding. Based on the previous findings, this study explored the expression patterns of STS genes VqSTS12, VqSTS24 and VqSTS25 through a variety of biotic and abiotic stress treatments. As the qRT-PCR results revealed, the VqSTS12, VqSTS24 and VqSTS25 genes could be induced and expressed significantly just after 12 h of powdery mildew induction. Besides, their second-highest expressions were found during 96~120 h. Additionally, the 3 STS genes could be expressed under the following stress treatments: methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), trauma, low temperature, salt and drought. This indicated that the VqSTS12, VqSTS24 and VqSTS25 genes could be broadly induced in response to biotic and abiotic stresses. Subcellular localization in leaves of Nicotiana benthamiana observations showed that these 3 STSs were localized in the cytoplasm of leaf epidermal cells. In Vitis vinifera cv. 'Thompson Seedless' overexpressing VqSTS12 and VqSTS25, significant up-regulations of STS genes (VqSTS12 and VqSTS25) were found in transgenic plants that were under drought stress simulated with 10% PEG6000. Moreover, the physiological indices like malondialdehyde (MDA) content, peroxidase (POD) activity and catalase (CAT) activity of the transgenic plants were all superior to the wild-type plants, which suggested that the over-expression of VqSTS12 and VqSTS25 genes improved the drought resistance of Thompson Seedless. In summary, the STS genes VqSTS12, VqSTS24 and VqSTS25 could be used as the disease-resistant germplasm resources of improved European grape varieties for resistance breeding. The results of the present study provide the candidate genes and referential basis for further improving the disease and stress resistances of Vitis vinifera.
吴凤颖, 刘梦琦, 王跃进. 中国野生毛葡萄3个芪合酶基因表达分析[J]. 农业生物技术学报, 2020, 28(5): 847-858.
WU Feng-Ying, LIU Meng-Qi, WANG Yue-Jin. Expression Analysis of Three Stilbene Synthase Genes from Chinese Wild Vitis quinquangularis. 农业生物技术学报, 2020, 28(5): 847-858.
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