Abstract：Calcineurin B-like interacting protein kinase (CIPK) is a class of serine/threonine (Ser/Thr) protein kinases widely existing in plants that are involved in decoding calcium signals. CIPKs play important roles in abiotic and biotic stress signal transduction. In this study, BnaCIPK2 and BnaCIPK16 genes were successfully cloned by reverse transcription-polymerase chain reaction (RT-PCR) from oilseed rape (Brassica napus). Sequence analysis showed that the 2 proteins encoded by the 2 BnaCIPK genes contained conserved kinase domain and asparagine-alanine-phenylalanine (NAF) motif. The subcellular localization analysis showed that both BnaCIPK2 and BnaCIPK16 were localized in both cytoplasm and nuclei. qRT-PCR profiling at 3 time points revealed that jasmonic acid (JA), cold and polyethylene glycol (PEG8000) significantly induced the expression of BnaCIPK2. After methyl viologen (MV) treatment for 24 h, the expression of BnaCIPK2 was inhibited. Salicylic acid (SA), heat, cold, hydrogen peroxide (H2O2) and PEG8000 up-regulated the expression of BnaCIPK16, while JA and abscisic acid (ABA) treatments resulted in the repression of BnaCIPK16 expression. Yeast two-hybrid (Y2H) screening and bimolecular fluorescence complementation (BiFC) showed that BnaCIPK2 and BnaCIPK16 interacted with Brassica napus calcineurin B-like protein 4 (BnaCBL4) and BnaCBL3, respectively. The present study provides basic information for elucidating the functions and regulatory mechanisms of BnaCIPK2 and BnaCIPK16.
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