Bioinformatics and Expression Characteristic Analysis of StUBC12 gene of Potato (Solanum tuberosum)
FU Xue1, TANG Xun1,2, LIU Wei-Gang2,3, LI Shi-Gui3, QI Xue-Hong1, ZHANG Ning1, SI Huai-Jun1,2,*
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 3 College of Agronomy , Gansu Agricultural University, Lanzhou 730070, China
Abstract：The ubiquitin-proteasome pathway is widely involved in various stages of plant growth and development. The ubiquitin-conjugating enzyme E2 is the key enzyme in the pathway of enzyme-catalyzed and plays an important role in process of substrate ubiquitination. In this study, the StUBC12 (GenBank No. XM_006343293.2) was cloned from the potato (Solanum tuberosun) cultivar 'Atlantic' leaf cDNA. The full-length of the gene was 2 112 bp, and the CDS region is 444 bp. It was a split gene containing 3 introns and 4 exons. Bioinformatics analysis of StUBC12 indicated that the gene was a non-transmembrane protein encoded by 148 amino acids with a typical UQ_con conserved domain. The qRT-PCR technology was used to analyze the gene expression level of roots, stems, leaves and flowers in potato cultivars 'Atlantic' and 'Qingshu 9'. The results showed that the gene expression level was the highest in leaves and flowers. The expression level in 'Atlantic' was significantly higher than 'Qingshu 9'. Under drought and salt stress, potato StUBC12 gene up-regulated to increase plant stress resistance. This study could provide a theoretical basis for further study of the potato stress-resistance mechanism and the functional of the StUBC12 gene.
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