Cloning and Expression Analysis of a ζ-carotene Desaturase Gene (HeZDS) in Hibiscus esculentus
LI Yong-Ping, WANG Bin, CHEN Min-Dong, ZHU Hai-Sheng*, WEN Qing-Fang*
Fujian Key Laboratory of Vegetable Genetics and Breeding / Crops Research Institute, Fujian Academy of Agricultural Sciences / Vegetable Research Center, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Abstract：ζ-carotene desaturase (ZDS) is the rate-limiting enzyme in carotenoid synthesis, catalyzing ζ-carotene to form lycopene. In this study, a ZDS gene was cloned from Hibiscus esculentus by transcriptome sequencing. The full-length cDNA sequence of ZDS was 2 189 bp, which contained a 1 686 bp ORF that encoded 561 amino acids, with a predicted molecular weight of 61.79 kD and a hypothetical isoelectric point (pI) of 6.807. It shared over 90% identity with the homologous proteins from Gossypium arboreum, G. hirsutum, Corchorus capsularis, Durio zibethinus and Theobroma cacao, showing that it was highly conservative. This gene was named HeZDS and the GenBank No. was MG372371. qRT-PCR analysis revealed that HeZDS could be expressed in different tissues of H. esculentus, including roots, stems, leaves, flowers, fruits and pod of Hibiscus esculentus, and the highest expression was found in mature leaves during leaf growth and tender pods (2 d after anthesis) in fruit development, respectively. Moreover, the expression of HeZDS gene had close correlations with carotenoid contents. These results provide a basis for the study of the HeZDS gene function and carotenoid regulation mechanism in H. esculentus.
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