Identification of NAC Gene Family and Its Response Analysis Under Drought Stress in Pisum sativum
LIU Fei1, YANG Qin-Kang1,2, HE De-Chuan1,2, XING Bao-Long3,*, LI Meng-Jiao1
1 Institute of High Latitude Crops, Shanxi Agricultural University, Datong 037000, China; 2 College of Agriculture, Shanxi Agricultural University, Jinzhong 030600, China; 3 Department of Social Service, Shanxi Agricultural University, Taiyuan 030000, China
Abstract：The NAC is one of the plant specific transcription factor and plays a key role in plant growth, development and stress responses. In this study, 84 NAC genes were identified from Pisum sativum genome database by sequence alignment using the NAC family genes of Arabidopsis thaliana and Glycine max. They were distributed unevenly on 7 chromosomes and divided into 13 subfamilies. All subfamily members had highly homologous sequences at the N-terminal region and highly divergent sequences at the C-terminal region. Members of the same subfamily had similar types and quantities of conserved motifs, and contain specific conserved motifs. Most NAC genes contained 3 exons. There were 29 and 50 NAC genes in the pea genome, which were collinear with the NAC genes in the Arabidopsis and soybean genome, respectively. Seventy-nine genes contained at least one abiotic stress related element such as drought responsive element and low-temperature responsive element. The drought stress expression profile showed that 60 PsNAC genes had obvious response to drought stress. 75.0% and 81.7% of the 60 PsNAC showed significant response in the early stage and late stage of drought stress, respectively, indicating that PsNAC gene played an important role in different stages of drought in peas. Among them, five PsNAC genes had higher response level in the shoot or root within 48 h of drought stress. This study analyzed the information of PsNAC gene family members which could provide a reference for further research on identification of stress resistance related genes.
刘飞, 杨亲康, 何德钏, 邢宝龙, 李梦蛟. 豌豆NAC基因家族鉴定及其在干旱胁迫下的响应分析[J]. 农业生物技术学报, 2024, 32(7): 1504-1517.
LIU Fei, YANG Qin-Kang, HE De-Chuan, XING Bao-Long, LI Meng-Jiao. Identification of NAC Gene Family and Its Response Analysis Under Drought Stress in Pisum sativum. 农业生物技术学报, 2024, 32(7): 1504-1517.
[1] 陈国户, 庞小可, 李广, 等. 2022. 白菜NAC基因家族全基因组鉴定及其应答春化反应的表达分析[J]. 南京农业大学学报, 45(4): 656-665. (Chen G H, Pang X K, Li G, et al.2022. Genome-wide identification of NAC gene family in Brassica rapa and its expression analysis of response to vernalization[J]. Journal of Nanjing Agricultural University, 45(4): 656-665.) [2] 李玲, 沈宝宇, 张天静, 等. 2017. 豌豆种质资源芽期耐旱性评价及耐旱种质筛选[J]. 植物遗传资源学报, 18: 778-785. (Li L,Shen B Y, Zhang T J, et al.2017. Evaluation and screening of pea (Pisum sativum) germplasm resources for drought resistance during germination stage[J]. Journal of Plant Genetic Resources, 18(4): 778-785.) [3] 王会文, 范军强, 路晓明, 等. 2022. 白菜型冬油菜NAC基因家族鉴定及表达分析[J]. 江苏农业学报, 38(5): 1315-1329. (Wang H W, Fan J Q, Lu X M, et al.2022. Identification and expression analysis of NAC gene family in Brassica rapa L[J]. Jiangsu Journal of Agriculture, 38(5):1315-1329.) [4] 文利超, 熊涛, 邓智超, 等. 2023. 烟草转录因子NtNAC080在非生物胁迫下的表达分析及功能鉴定[J]. 作物学报, 49(08): 1-13. (Wen L C, Xiong T, Deng Z C, et al.2023. Expression and functional characterization of NtNAC080 transcription factor gene from Nicotiana tabacumin under abiotic stress[J]. Journal of Crop Science, 49(08): 1-13.) [5] Balakrishnan S, Shenghan G, J L M, et al.2019. Evolview v3: A webserver for visualization, annotation, and management of phylogenetic trees[J]. Nucleic Acids Research, 47(1): 270-275. [6] Chao J T, Li Z Y, Sun Y H, et al.2021. MG2C: A user-friendly online tool for drawing genetic maps[J]. Molecular Horticulture, 1(1): 16. [7] Chen C J, Chen H, Zhang Y, et al.2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant, 13(8): 1194-1202. [8] Chun I, Kim H J, Hong S, et al.2022. Structural basis of DNA binding by the NAC transcription factor ORE1, a master regulator of plant senescence[J]. Plant Communications, 4(3): 100510. [9] Du L Y, Huang X L, Ding L, et al.2023. TaERF87 and TaAKS1 synergistically regulate TaP5CS1/TaP5CR1-mediated proline biosynthesis to enhance drought tolerance in wheat[J]. New Phytologist, 237(1): 232-250. [10] Dwivedi V, Pal L, Singh S, et al.2023. The chickpea WIP2 gene underlying a major QTL contributes to lateral root development[J]. Journal of Experimental Botany, 75(2): 642-657. [11] Fang Y, You J, Xie K, et al.2008. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice[J]. Molecular Genetics and Genomics, 280: 547-563. [12] Han K J, Zhao Y, Sun Y H, et al.2023. NACs, generalist in plant life[J]. Plant Biotechnology Journal, 21(12): 2433-2457. [13] Hou L X, Zhu D, Ma Q, et al.2016. H2S synthetase AtD-CDes involves in ethylene and drought regulated stomatal movement[J]. Science Bulletin, 61(15): 1171-1175 [14] Jensen M K, Kjaersgaard T, Nielsen M M, et al.2010. The Arabidopsis thaliana NAC transcription factor family: Structure-function relationships and determinants of ANAC019 stress signalling[J]. Biochemical Journal, 426(2): 183-196. [15] Krzywinski M, Schein J, Birol I, et al.2009. Circos: An information aesthetic for comparative genomics[J]. Genome Research, 19(9): 1639-1645. [16] Li W J, He X, Chen Y, et al.2020. A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal[J]. New Phytologist, 225(4): 1667-1680. [17] Li Y X, Han S C, Sun X M, et al.2023. Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice[J]. Journal of Integrative Plant Biology, 65(4): 918-933. [18] Liu Q, Yan S J, Huang W J, et al.2018. NAC transcription factor ONAC066 positively regulates disease resistance by suppressing the ABA signaling pathway in rice[J]. Plant Molecular Biology, 98(4-5): 289-302. [19] Mao H D, Li S M, Chen B, et al.2022. Variation in cis-regulation of a NAC transcription factor contributes to tolerance in wheat[J]. Molecular Plant, 15(2): 276-292. [20] Mao H D, Yu L J, Han R, et al.2016. ZmNAC55, a maize stress-responsive NAC transcription factor, confers drought resistance in transgenic Arabidopsis[J]. Plant Physiology and Biochemistry, 105: 55-66. [21] Melo B P, Fraga O T, Silva J C F, et al.2018. Revisiting the soybean GmNAC superfamily[J]. Frontiers in Plant Science, 9: 1864. [22] Min X Y, Jin X Y, Zhang Z S, et al.2020. Genome-wide identification of NAC transcription factor family and functional analysis of the abiotic stress-responsive genes in Medicago sativa L[J]. Journal of Plant Growth Regulation, 39(1): 324-337. [23] Olsen AN, Ernst HA, Leggio LL, et al.2005. NAC transcription factors: Structurally distinct, functionally diverse[J]. Trends in Plant Science, 10(2): 79-87. [24] Ooka H, Satoh K, Doi K, et al. 2003. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J]. DNA Research, 10(6): 239-247. [25] Perochon A, Kahla A, Vranic M, et al.2019. A wheat NAC interacts with an orphan protein and enhances resistance to Fusarium head blight disease[J]. Plant Biotechnology Journal, 17(10): 1892-1904. [26] Puranik S, Sahu P P, Srivastava P S, et al.2012. NAC proteins: regulation and role in stress tolerance[J]. Trends in Plant Science, 17(6): 369-381. [27] Saidi MN, Mergby D, Souibgui A, et al.2022. Overexpression of durum wheat NAC transcription factor TtNTL3A promotes early flowering and increases multiple stress tolerance in transgenic Arabidopsis[J]. Plant Physiology Biochemistry, 192: 1-9. [28] Sakuraba Y, Kim Y S, Han S H, et al.2015. The Arabidopsis transcription factor NAC016 promotes drought stress responses by repressing AREB1 transcription through a trifurcate feed-forward regulatory loop involving NAP[J]. Plant Cell, 27(6): 1771-1787. [29] Song S, Willems L A J, Jiao A, et al.2022. The membrane associated NAC transcription factors ANAC060 and ANAC040 are functionally redundant in the inhibition of seed dormancy in Arabidopsis thaliana[J]. Journal of Experimental Botany, 73(16): 5514-5528. [30] Sun X M, Xiong H Y, Jiang C H, et al.2022. Natural variation of DROT1 confers drought adaptation in upland rice[J]. Nature Communications, 13(1): 4265. [31] Tamura K, Peterson D, Peterson N, et al.2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 28(10): 2731-2739. [32] Tariq R, Hussain A, Tariq A, et al.2022. Genome-wide analyses of the mung bean NAC gene family reveals orthologs, co-expression networking and expression profiling under abiotic and biotic stresses[J]. BMC Plant Biology, 22(1): 343. [33] Tran L S P, Nakashima K, Sakuma Y, et al.2004. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter[J]. The Plant Cell, 16(9): 2481-2498. [34] Wang Y P, Tang H B, DeBarry J D, et al.2012. MCScanX: A toolkit for detection and evolutionary analysis of gene synteny and collinearity[J]. Nucleic Acids Research, 40(7): e49. [35] Xie Q, Frugis G, Colgan D, et al.2000. Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development[J]. Genes & Development, 14(23): 3024-3036. [36] Yang C F, Huang Y Z, Lv P Y, et al.2022. NAC transcription factor GmNAC12 improved drought stress tolerance in soybean[J]. International Journal of Molecular Sciences, 23(19): 12029. [37] Yarra R, Wei W.2021. The NAC-type transcription factor GmNAC20 improves cold, salinity tolerance, and lateral root formation in transgenic rice plants[J]. Functional & Integrative Genomics, 21(3-4): 473-487. [38] Zhang Q, Luo F, Zhong Y, et al.2020. Modulation of NAC transcription factor NST1 activity by XYLEM NAC DOMAIN1 regulates secondary cell wall formation in Arabidopsis[J]. Journal of Experimental Botany, 71(4): 1449-1458. [39] Zhang Y J, Geng H M, Cui Z C, et al.2021. Functional analysis of wheat NAC transcription factor, TaNAC069, in regulating resistance of wheat to leaf rust fungus[J]. Frontiers in Plant Science, 12: 604797. [40] Zhang Z G, Lv G D, Li B, et al.2017. Isolation and characterization of the TaSnRK2.10 gene and its association with agronomic traits in wheat (Triticum aestivum L.)[J]. PLOS One, 12(3): e0174425. [41] Zheng Y C, Jin X Y, Wang J Y, et al.2023. SbNAC9 improves drought tolerance by enhancing scavenging ability of reactive oxygen species and activating stress-responsive genes of sorghum[J]. International Journal of Molecular Sciences, 24(3): 2401.
