Identification of the SNAC Gene Family in Eucalyptus grandis and Its Expression Analysis Under Abiotic Stresses
HONG Jia-Du, NI Xiao-Xiang, YU Jian-Feng, WU Meng-Jie, ZHAO Shuang, CHENG Long-Jun*
State Key Laboratory of Subtropical Silviculture/College of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
Abstract：Eucalyptus grandis has made important contribution to wood-related industries in South China, but they are sensitive to abiotic stresses, limiting the expansion of its cultivation and benefits improvement of plantation. NAC is a plant-specific transcription factor family, which is widely involved in plant growth, development, metabolism and stress response. And, the stress-related NACs were named SNAC. In this study, 166 NAC transcription factors identified in E. grandis genome and 74 SNAC in other plant species were used to identify EgrSNACs. And, EgrSNACs structure, coding protein sequence, chromosome localization, collinearity and gene duplication, cis-acting elements on promoters, and the tissue-specific expression patterns were analyzed. In addition, the seedlings of E. grandis were treated with low temperature, drought, high salt, abscisic acid (ABA) and methyl jasmonate (MeJA), and qPCR was used to analyze the expression of EgrSNACs under these treatments. The results showed that there were 22 EgrSNACs in E. grandis, belonging to ATAF, NAP, and AtNAC3 subfamily. Except for EgrSNAC20, the proteins encoded by these genes all contained 5 subdomains of the typical NAM domain, corresponding to Motif1~Motif5. The 22 EgrSNACs were distributed on 9 chromosomes, with 4 collinear gene pairs and 2 tandem repeat gene segments composed of 11 EgrSNACs. Cis-acting element of promoter analysis showed that multiple stress response elements were distributed on the EgrSNAC promoters. The expression pattern of tandem duplicated EgrSNACs were similar in leaf, stem, xylem and phloem. The qPCR analysis of different time treatments at 4 ℃ indicated that the expression of the 19 EgrSNACs were induced by low temperature, in addition to EgrSNAC1, EgrSNAC3, and EgrSNAC22; there were 19 EgrSNACs that responded to the drought, except EgrSNAC2, EgrSNAC5, EgrSNAC14 and EgrSNAC21 were suppressed, others were induced. Under high salt treatment, 19 EgrSNACs expression changes, and only EgrSNAC21 was suppressed. In addition, 14 EgrSNACs were induced under ABA (100 μmol/L) treatment. 13 EgrSNACs were induced and 4 were inhibited under MeJA (100 μmol/L) treatment . The results of this study revealed the relationship between EgrSNACs and their response of low temperature, drought, high salt, ABA, MeJA and other abiotic stress factors, providing a reference for further study of EgrSNACs in E. grandis.
洪家都, 倪晓祥, 俞键烽, 吴梦洁, 赵爽, 程龙军. 巨桉SNAC基因家族的鉴定及其在非生物逆境下的表达分析[J]. 农业生物技术学报, 2024, 32(1): 115-131.
HONG Jia-Du, NI Xiao-Xiang, YU Jian-Feng, WU Meng-Jie, ZHAO Shuang, CHENG Long-Jun. Identification of the SNAC Gene Family in Eucalyptus grandis and Its Expression Analysis Under Abiotic Stresses. 农业生物技术学报, 2024, 32(1): 115-131.
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