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2025年4月2日 星期三
农业生物技术学报  2024, Vol. 32 Issue (7): 1518-1532    DOI: 10.3969/j.issn.1674-7968.2024.07.005
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
姜OSCA基因家族的鉴定及表达分析
龙茶1,2,3, 汪淘1,2, 花河兰4, 吴媛1,2, 苏世贤1, 韦金江1,2, 江苏燕1, 牟海林1,2, 祝洪江1,2, 吕金丽5, 夏应书6, 李伟1,2,3,*
1 贵州大学 农学院,贵阳 550025;
2 贵州大学 蔬菜研究院,贵阳 550025;
3 贵州省高等学校 设施蔬菜工程研究中心,贵阳 550025;
4 花溪区植保站,贵阳 550025;
5 六盘水市农业科学研究院,六盘水 553000;
6 贵州黔棠姜生物科技有限公司,安顺 561200
Identification and Expression Analysis of OSCA Gene Family in Ginger (Zingiber officinale)
LONG Cha1,2,3, WANG Tao1,2, HUA He-Lan4, WU Yuan1,2, SU Shi-Xian1, WEI Jin-Jiang1,2, JIANG Su-Yan1, MOU Hai-Lin1,2, ZHU Hong-Jiang1,2, LYU Jin-Li5, XIA Ying-Shu6, LI Wei1,2,3,*
1 College of Agriculture, Guizhou University, Guiyang 550025, China;
2 Vegetable Research Institute, Guizhou University, Guiyang 550025, China;
3 Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions, Guiyang 550025, China;
4 Huaxi District Plant Protection Station, Guiyang, 550025, China;
5 Liupanshui Academy of Agricultural Sciences, Liupanshui 553000, China;
6 Guizhou Qiantangjiang Biotechnology Co., Ltd., Anshun, 561200 China
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摘要 钙通透性阳离子通道蛋白(hyperosmolarity-gate calcium-permeable channels, OSCA)是一种机械敏感性成孔离子通道,能调节渗透胁迫下植物细胞内的Ca2+浓度以及水分运输速率,在植物的高渗应激中起着重要作用。为了解OSCA基因家族在姜(Zingiber officinale)中的结构特征和表达特点,本研究对姜OSCA基因家族进行鉴定与表达分析。结果表明,从姜基因组中鉴定到了38个OSCA基因家族成员,分别定位于14条染色体上。亚细胞定位预测表明,姜OSCA家族成员主要定位于质膜上。启动子顺式作用元件分析显示,38个ZoOSCAs含有茉莉酸甲酯、脱落酸以及干旱等多种于逆境和激素应答响应元件。盐胁迫转录组及干旱处理的荧光定量结果显示,盐胁迫下叶和根茎中分别有18和23个家族成员表达量上调;干旱处理下14个成员中有11个表达量上调,且ZoOSCAs在不同组织中的表达水平不同。本研究可为深入揭示ZoOSCAs的功能和作用机制提供基础资料。
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龙茶
汪淘
花河兰
吴媛
苏世贤
韦金江
江苏燕
牟海林
祝洪江
吕金丽
夏应书
李伟
关键词 钙通透性阳离子通道蛋白(OSCA)基因家族鉴定表达分析    
Abstract:Hyperosmolarity gate calcium-permeable channels (OSCA) is a mechanosensitive porous ion channel, which can regulate Ca2+ concentration and water transport rate in plant cells under osmotic stress. It plays an important role in hypertonic stress in plants. In order to understand the structural characteristics and expression characteristics of OSCA gene family in ginger, this study identified and analyzed the OSCA gene family in ginger (Zingiber officinale). The results showed that 38 members of OSCA gene family were identified from ginger genome, which were located on 14 chromosomes respectively. The prediction of subcellular localization shows that the members of OSCA family in ginger were mainly located in plasma membrane. The analysis of cis-acting elements of promoters showed that 38 ZoOSCAs contained various response elements to adversity and hormone, such as methyl jasmonate, abscisic acid and drought. The transcriptome of salt stress and fluorescence quantitative results of drought treatment showed that the expression levels of 18, 23 family members in leaves and rhizomes were up-regulated under salt stress, and 11 out of 14 family members were up-regulated under drought treatment, and the expression levels of ZoOSCAs in different tissues were different. This study provides basic data for revealing the function and mechanism of ZoOSCAs.
Key wordsGinger    Hyperosmolarity-gate calcium-permeable channels (OSCA)    Gene family    Identification    Expression analysis
收稿日期: 2023-07-07     
中图分类号: S632.5
基金资助:贵州省高等学校设施蔬菜工程研究中心平台建设项目(黔教技[2022]040号),贵州省农业农村厅生姜小分队(第一批农村产业革命)蔬菜产业发展项目(黔财农[2020]98号); 农业农村部/贵州省农业农村厅镇宁县2022年贵州高原夏秋蔬菜优势特色产业集群项目(黔财农[2022]89号)
通讯作者: * wli@gzu.edu.cn   
引用本文:   
龙茶, 汪淘, 花河兰, 吴媛, 苏世贤, 韦金江, 江苏燕, 牟海林, 祝洪江, 吕金丽, 夏应书, 李伟. 姜OSCA基因家族的鉴定及表达分析[J]. 农业生物技术学报, 2024, 32(7): 1518-1532.
LONG Cha, WANG Tao, HUA He-Lan, WU Yuan, SU Shi-Xian, WEI Jin-Jiang, JIANG Su-Yan, MOU Hai-Lin, ZHU Hong-Jiang, LYU Jin-Li, XIA Ying-Shu, LI Wei. Identification and Expression Analysis of OSCA Gene Family in Ginger (Zingiber officinale). 农业生物技术学报, 2024, 32(7): 1518-1532.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.07.005     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I7/1518
 
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