Cloning, Expression Analysis and Subcellular Localization of PsDXR and PsMCS Genes in Tree Peony (Paeonia suffruticosa)
LI Zi-Yao1, LIU Bing1, HU Zeng-Hui1,2, WU Jing1,2*, LENG Ping-Sheng1,2*
1 College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206; 2 Beijing Laboratory of Urban and Rural Ecological Environment, Beijing University of Agriculture, Beijing 102206
Abstract：Terpenoids are the main floral components of tree peony (Paeonia suffruticosa). 1-deoxyxylulose-5- phosphate reductoisomerase (DXR) and 2-C-methylerythritol-2, 4-cyclodiphosphate synthase (MCS) play important roles as key enzymes in the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway in plant terpene synthesis. To investigate the role of PsDXR and PsMCS genes in the terpene synthesis pathway of tree peony, the PsDXR (GenBank No. ON092613) and PsMCS genes (GenBank No. ON092614) were cloned using the 'Huang Guan' cDNA of tree peony in this study, and their bioinformatics analysis, subcellular localization and qRT-PCR were performed to analyze the expression in different flower developmental stages and tissues of tree peony 'Huang Guan'. The results showed that the cDNAs of PsDXR and PsMCS genes were 1 401 and 702 bp in length, encoding 466 and 233 amino acids, respectively. The proteins encoded by the 2 genes were stable hydrophilic proteins. The PsDXR protein had the conserved domains of DXP_reductoisom and DXP_redisom_C, and the PsMCS protein had the MECDP-specific domain. The results of subcellular localization showed that both proteins were localized in the chloroplast. The expression patterns of PsDXR and PsMCS genes both increased and then decreased in flower development stages; both 2 genes were differentially expressed in different tissues (P<0.05). The results indicated that PsDXR and PsMCS genes might play important roles in the MEP metabolic pathway of tree peony, provides a molecular basis for the biosynthesis of terpenes in tree peony.
李紫瑶, 刘冰, 胡增辉, 吴静, 冷平生. 牡丹PsDXR和PsMCS基因的克隆、表达分析及亚细胞定位[J]. 农业生物技术学报, 2023, 31(4): 730-740.
LI Zi-Yao, LIU Bing, HU Zeng-Hui, WU Jing, LENG Ping-Sheng. Cloning, Expression Analysis and Subcellular Localization of PsDXR and PsMCS Genes in Tree Peony (Paeonia suffruticosa). 农业生物技术学报, 2023, 31(4): 730-740.
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