1 College of Horticulture, Shanxi Agricultural University, Taigu 030801, China; 2 College of Information Science and Engineering, Shanxi Agricultural University, Taigu 030801, China; 3 Shanxi Key Laboratory of Germplasm Improvement and Utilization in Pomology, Shanxi Agricultural University, Taigu 030801, China
Abstract：WRKY family is widely involved in plant growth and development, regulating substance metabolism, responding to environmental signals and so on. To study the function of grape (Vitis vinifera) VvWRKY26, the VvWRKY26 gene (GenBank No. KX 823961.1) was cloned from 'WuHeZaoHong' grape by homologous sequence method, the bioinformatics, spatio-temporal expression and response salicylic acid (SA) expression were analyzed. The results of bioinformatics analysis showed that the CDS region of VvWRKY26 was 1 434 bp length, encoded 477 amino acids, and its secondary structure was composed of random curl, α-helix and β-fold, which was a non-secreted, non-transmembrane protein distributed in the nucleus. It was highly homologous with Petunia hybrid PhPH3 (GenBank No. AMR43368.1), Brassica napus BnTTG2 (Brassica napus transparent testa glabra2, GenBank No. 106423067) and Arabidopsis thaliana AtTTG2 (Arabidopsis thaliana transparent testa glabra2, GenBank No. 818303), and belongs to the WRKY transcription factor family Ⅰ. Promoter analysis showed that VvWRKY26 promoter had response elements such as light signal, resistance to reactive oxygen stress, SA and gibberellin acid (GA) signals, etc.. qPCR analysis showed that VvWRKY26 was expressed in the flowers, stems, buds, tendrils, young leaves, mature leaves and fruit tissues, and the expression levels were higher during the young fruit stage and color changing stage. It participated in SA signal response and the expression level under the SA treatment at 0.5~8 h was significantly higher than that of the control (P<0.05). This study provides a theoretical basis for the further research on the function of VvWRKY26 in SA signal channel.
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