Analysis of Differential Expression Genes Related to Organic Acid in Fruits of Two Citrus Cultivars
WANG Jian-Hui1*, YANG Xin2, GONG Xiao-Yuan1, YU Jie-Lin1, HAN Dong-Mei1, LI Lin2, LI Xiang1, LIU Da-Yu1, LI Jing-Jing3, TANG Jiang4
1 College of Food and Biological Engineering, Chengdu University, Chengdu 610000, China; 2 College of Ecology and Environment, Chengdu University of Technology, Chengdu 610000, China; 3 The Sixth People's Hospital of Chengdu, Chengdu 610000, China; 4 Anyue Lemon Science and Technology Institute, Anyue County 642350, China
Abstract：Fruit flesh of Citrus is rich in citrate and other nutrients. To better understand the effects of differential expression of genes (DEGs) on citrate and other important metabolites in Citrus, both fruit qualities and RNA-seq analysis were performed between 'Sweet lemon' and 'Eureka', respectively. The comparative analysis between 2 cultivars had been implemented using transcriptomics technology to identify the DEGs, which caused citrate accumulating in pulp. After transcriptomic sequencing for each fruit in 3 replicates, 73 678 unigenes had been obtained, out of which the total 4 044 unigenes were differentially expressed between 2 cultivars. Subsequently, DEGs were further enriched into different pathways by KEGG. The upregulating DGEs in 'Sweet lemon' with lower citrate contents were enriched into 24 pathways, including GABA shunt. Besides, upregulating DEGs in 'Eureka' with higher citrate contents were enriched into 13 pathways, including citrate cycle. At fruit ripening stage, the significantly differential expression of citrate synthase was not found between 2 cultivars. In addition, proton pump gene in 'Eureka' had higher transcription levels than 'Sweet lemon' at fruit ripening stage, so that it was benefit for vacuole acidification in fruit. It could be concluded that up-regulating expression of genes involved into citrate metabolism and proton transporting were correlated with organic acidity accumulations in Citrus fruits. This study provides an insight into organic acids metabolism in Citrus and is useful for breeding program in future.
王建辉, 杨鑫, 龚晓源, 余洁霖, 韩冬梅, 李凛, 李翔, 刘达玉, 李京晶, 唐江. 两个柑橘品种果实有机酸相关基因的差异表达分析[J]. 农业生物技术学报, 2023, 31(4): 718-729.
WANG Jian-Hui, YANG Xin, GONG Xiao-Yuan, YU Jie-Lin, HAN Dong-Mei, LI Lin, LI Xiang, LIU Da-Yu, LI Jing-Jing, TANG Jiang. Analysis of Differential Expression Genes Related to Organic Acid in Fruits of Two Citrus Cultivars. 农业生物技术学报, 2023, 31(4): 718-729.
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