Identification of MYB Gene Family and Its Expression Pattern Analysis Under Different Light Quality in Spine Grape (Vitis davidii)
QUE Qiu-Xia1,2, LAI Gong-Ti2, PAN Ruo1,2, ZHANG Jing1,2, HE Li-Yuan1,2, LAI Pu-Fu2, WANG Qi2, GAO Hui-Ying2, CHEN Gui-Xin1,*, LAI Cheng-Chun2,*
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
Abstract：MYB transcription factor is an important regulatory factor in plant growth and secondary metabolism, and plays key roles in anthocyanin biosynthesis. In this research, 9 VdMYB genes were identified and cloned from Vitis davidii callus based on previous transcriptome data. The MYB gene structure and function were analyzed and predicated by bioinformatic softwares, and their expression patterns were analyzed under different light quality. The results showed that most MYB genes included 3 exons and 2 introns; the number of amino acid residues of VdMYBs were 216~335, the relative molecular mass were 24.09~36.17 kD, and the isoelectric point were 5.42~9.58. The phylogenetic tree analysis indicated that the 9 VdMYB proteins were divided into 6 groups, and were predicted to be located in cytoplasm, mitochondria and nucleus, respectively. Analysis of promoter cis-acting elements showed that a large number of light-response and bio/abio-response elements were predicted in the 9 VdMYB promoters. The qRT-PCR analysis showed that light quality significantly affected the expression of VdMYBs, their expression pattern corresponded to the structural characteristics under different light quality. Among 9 VdMYB genes, VdMYB31/VdMYBB1 and VdMYB4A might play as positive and negative regulators involved in anthocyanin biosynthesis.This study provides theoretical reference for further elucidating the light-response molecular mechanism of MYB family genes involved in the regulatory pathways of anthocyanins in Vitis davidii.
阙秋霞, 赖恭梯, 潘若, 张静, 贺丽媛, 赖谱富, 王琦, 高慧颖, 陈桂信, 赖呈纯. 刺葡萄MYB基因家族鉴定及其在不同光质下的表达模式分析[J]. 农业生物技术学报, 2023, 31(2): 298-310.
QUE Qiu-Xia, LAI Gong-Ti, PAN Ruo, ZHANG Jing, HE Li-Yuan, LAI Pu-Fu, WANG Qi, GAO Hui-Ying, CHEN Gui-Xin, LAI Cheng-Chun. Identification of MYB Gene Family and Its Expression Pattern Analysis Under Different Light Quality in Spine Grape (Vitis davidii). 农业生物技术学报, 2023, 31(2): 298-310.
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