Bioinformatics and Expression Analysis of StERF5 Gene in Potato (Solanum tuberosum)
WANG Rui1, PU Xue1, WANG Kai-Tong1, ZHANG Huan-Huan1,2, ZHANG Ning1,2,*, SI Huai-Jun1,2
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China
Abstract：APETALA2/ethylene responsive factor (AP2/ERF) family is one of the largest transcription factor families in plants. ERF subfamily is the main member of AP2/ERF family and plays an important role in plant response to biotic and abiotic stresses. In this experiment, the bioinformatics analysis, subcellular localization and gene expression analysis of potato (Solanum tuberosum) StERF5 gene were carried out to further clarify the function of the gene. The results showed that the coding region of the gene was encoding 243 amino acids and contained an AP2 domain. The subcellular localization showed that the protein was localized in the nucleus and cell membrane. Tissue specific expression analysis showed that StERF5 gene was expressed in roots, stems and leaves, with the highest expression level in roots. There were significant differences in the expression levels of StERF5 gene in stems of different potato varieties (P<0.01). Expression of StERF5 gene was up-regulated under drought and salt treatments. This study lays the foundation for the subsequent in-depth understanding of function of StERF5.
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