Cloning and Expression Analysis of Flowering-related Gene VrFT2a in Mung Bean (Vigna radiata)
YU Jing-Jing1, LI Meng-Xin1, CAI De-Bao1, LIU Jia-Fei3, ZHANG Jun1, ZHANG Wan2, CAO Ling-Ling1, CHEN Ji-Bao1, YANG Shu-Qing1,*
1 Henan Provincial Collaborative Innovation Center for Water Safety, Nanyang 473061, China; 2 College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China; 3 Key Laboratory of Insect Biology in Funiu Mountain, Nanyang 473061, China
Abstract：Mung bean (Vigna radiata) is the main coarse grain crop in China, and the flowering time plays an important impact on the production, quality and fertile period of mung bean. FLOWERING LOCUS T (FT) genes play an important role in the flowering pathway of plant, which are also the key genes in the flowering regulatory network. In order to clarify the regulatory role of FT family homologous genes in the photoperiod-related flowering of mung bean, the cDNA sequence of VrFT2a (GenBank NO. XM_014651110) was cloned from the photoperiod-sensitive mung bean variety 'Xinzheng'. The coding region, encoded proteins characteristics, and its expression patterns of VrFT2a in different tissues and photoperiod conditions were analyzed by bioinformatics. Nine light responsive cis-acting elements were found in the promoter region of VrFT2a, the full-length of cDNA was 531 bp, which encoding 176 amino acids wherein the 27th to 162th amino acids were highly conserved PEBP (phosphatidylethanolamine-binding protein) family domain. The encoding protein of VrFT2a was a non-secretory hydrophilic protein with none signaling peptide. Subcellular localization analysis indicated that VrFT2a localized in plasma membrane and nucleus. Phylogenetic analysis showed that the amino acids of VrFT2a had a higher homology with other FTs. VrFT2a, GmFT2a, and AtFT had similar three-dimensional protein structures, and the conserved flowering-related amino acids of FT (Tyr85/Gln140). qRT-PCR analyses revealed that VrFT2a was expressed in different tissues, with its highest expression in trifoliolates of mung bean. The expression level of VrFT2a exhibited an obvious diurnal rhythm under long-day and short-day conditions, and it also was specifically induced by the short-day conditions significantly. The results implicated that VrFT2a might involve in the photoperiod regulatory pathway of mung bean. This study will provide a theoretical reference for further deconstruction of the molecular mechanisms of FT family genes in photoperiodic regulation pathways of mung bean.
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