Cloning, Expression and Protein Interaction Analysis of Drought-responsive Gene StGAMyb-like1 in Potato (Solanum tuberosum)
DENG Yu-Rong1,2, WEI Jing-Jing1,2, LIU Sheng-Yan1,3, YANG Liang1,3, WANG Xiao-Feng1,3, YANG Jiang-Wei1,2, ZHANG Ning1,2, SI Huai-Jun1,2,*
1 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
Abstract：R2R3-MYB transcription factors, as the largest subfamily of the MYB transcription factor family, play a crucial role in plant growth and development, material metabolism, and other processes. Additionally, they have the potential to enhance plant stress tolerance. In a previous study, a StGAMyb-like1 gene (GenBank No. XM_015303144), homologous to AtMYB33 in Arabidopsis thaliana, was identified in potato (Solanum tuberosum). The StGAMyb-like1 gene, a member of GAMYB subgroup 18 of R2R3-MYB, is capable of responding to drought stress. In this study, the StGAMyb-like1 gene was cloned from the potato variety 'Desiree', and its CDS was 1 572 bp. Bioinformatics analysis revealed that the promoter region of this gene contained multiple hormone response elements, and its protein sequence was similar to that of tomato (Solanum lycopersicum). The expression analysis in different tissues showed that the StGAMyb-like1 gene had higher expression level in leaves. The promoter sequence of the StCYP98A3 gene, which encodes coumarate 3-hydroxylase in the lignin synthesis pathway, was analyzed and found to have MYB binding site (MBS). It was confirmed that StGAMyb-like1 binded to the StCYP98A3 promoter and activated its transcription using yeast one hybrid, dual-luciferase reporter assay and β-glucuronidase (GUS) detection. This study provides a reference basis for further exploring the function of StGAMyb-like1 gene.
邓玉荣, 魏晶晶, 刘升燕, 杨亮, 王晓凤, 杨江伟, 张宁, 司怀军. 马铃薯干旱响应基因StGAMyb-like1克隆、表达及蛋白互作分析[J]. 农业生物技术学报, 2024, 32(11): 2474-2483.
DENG Yu-Rong, WEI Jing-Jing, LIU Sheng-Yan, YANG Liang, WANG Xiao-Feng, YANG Jiang-Wei, ZHANG Ning, SI Huai-Jun. Cloning, Expression and Protein Interaction Analysis of Drought-responsive Gene StGAMyb-like1 in Potato (Solanum tuberosum). 农业生物技术学报, 2024, 32(11): 2474-2483.
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