StWIP2 Regulates the Resistance to Phytophthora infestans in Potato (Solanum tuberosum)
ZHU Di1, ZHOU Yun1,2,3, YANG Sheng-Long1,2,3, SUN Hai-Hong1,2,3, HE Miao-Miao1,2,3,*
1 Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China; 2 Key Laboratory of Qinghai-Tibetan Plateau Biotechnology (Qinghai University), Ministry of Education, Xining 810016, China; 3 Key Laboratory of Potato Breeding, Qinghai Province/Ministry of Education, Engineering Research Center of Potato in Northwest Region, Xining 810016, China
Abstract：Wound-induced proteins (WIPs) play an essential role in plants defence responses to pathogen infestation. In this study, the StWIP2 gene (GenBank No. NM_001288699.1) was cloned from potato (Solanum tuberosum) 'Qingshu 9', and bioinformatics analyses of the StWIP2 gene were carried out, while overexpression vectors and silencing vectors were constructed, and reverse genetics was used to characterise the function of the StWIP2 gene in the interaction with Phytophthora infestans. Bioinformatics revealed that the StWIP2 gene had a full CDS length of 636 bp and encoded a total of 211 amino acids. qPCR analysis revealed that the StWIP2 gene had the highest expression in 'Chunshu 5' and the lowest expression in diploid potato DM. The StWIP2 gene was responsive and differentially expressed under treatments of P. infestans, salicylic acid (SA), abscisic acid (ABA), and methyl jasmonate (MeJA). Overexpression of StWIP2 was able to increase the content of H2O2 and malondialdehyde (MDA), the activity of antioxidant enzymes, and the expression levels of pathogenesis related protein 1 (StPR1), StPR5 and ethylene responsive factor 1 (StERF1), to delay the onset of the disease in the plants and to increase the level of plant resistance to P. infestans. After silencing the StWIP2 gene, the content of H2O2 and MDA in plants decreased, and the activity of antioxidant enzymes decreased; The expression levels of plant defense gene 1.2 (PDF1.2), StERF1, peroxidase gene (StPOD), and phenylalanine ammonia lyase (StPAL) were reduced, while the expression of StPR1 and StPR5 were inhibited, resulting in a decrease in plant resistance to late blight. The above results indicate that the StWIP2 gene plays a positive regulatory role in the interaction with P. infestans, and the StWIP2 gene can be utilized as a candidate genetic resource for disease resistance breeding in potatoes.
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