草莓Trihelix转录因子家族的鉴定及表达分析

doi:10.3969/j.issn.1674-7968.2024.09.006

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摘要 Trihelix是能与光应答元件GT元件进行特异性结合的转录因子,参与植物的生长发育及逆境胁迫响应。本研究从草莓(Fragaria vesca)全基因组范围内识别Trihelix转录因子家族成员,并对其进行了生物信息学和逆境胁迫下的表达分析。结果显示,草莓Trihelix转录因子家族包含26个成员,分布于chr01~chr07染色体上,其中,chr06染色体上分布最多,有7个Trihelix转录因子家族成员;亚细胞定位预测结果显示,FvTrihelix转录因子家族成员主要定位在细胞核和细胞质;系统进化分析表明,Trihelix蛋白分为5个亚家族,其中Group2、Group3、Group4和Group5分别包含1、3、11和11个FvTrihelix成员。顺式作用元件分析表明,该家族转录因子可能参与激素、干旱和低温胁迫;qRT-PCR结果表明,4 ℃处理下,FvTrihelix14相对表达量均显著高于对照(P<0.05),是对照的8倍,FvTrihelix15的表达量在10% PEG和4 ℃处理下与对照相比差异显著(P<0.05),分别是对照的12和14倍。综上所述,草莓Trihelix转录因子家族成员能够响应不同的逆境胁迫。本研究为草莓抗逆基因的筛选和应用提供了理论依据。

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	陆洋
	张志强
	卢世雄
	毛娟
	陈佰鸿

关键词 ：草莓, Trihelix转录因子家族, 生物信息学, 表达分析

Abstract：Trihelix are transcription factors that can specifically bind to the light-responsive element GT element, and are widely involved in plant growth and development and response to adversity stress. In this study, the Trihelix transcription factor family members were identified in the strawberry (Fragaria vesca) genome, and further conducted bioinformatics and expression analysis under stress. The results showed that the strawberry Trihelix transcription factor family consisted of 26 members, distributed on chr01~chr07, among them, the largest number of members were distributed on chr06, with 7 Trihelix transcription factor family members. The results of the subcellular localization prediction showed that the FvTrihelix transcription factor family members were mainly localized in the nucleus and cytoplasm. Phylogenetic analysis showed that FvTrihelix proteins were divided into 5 subfamilies, of which Group2, Group3, Group4 and Group5, contained 1, 3, 11 and 11 FvTrihelix members, respectively. Cis-acting element analysis showed that the members of this family were mainly related to exogenous hormones, drought and low temperature response elements. qRT-PCR results showed that the relative expression of FvTrihelix14 were significantly higher than the control under 4 ℃ condition (P<0.05), being 8 times that of the control (CK). The expression level of FvTrihelix15 under 10% PEG and 4 ℃ treatments showed significant differences compared to the CK (P<0.05), being 12 and 14 times that of the control, respectively. In summary, the members of the strawberry Trihelix transcription factor family are able to respond to various stress conditions. This study provides a theoretical basis for the screening and application of stress-resistant genes in strawberries.

Key words： Strawberry Trihelix transcription factor family Bioinformatics Expression analysis

收稿日期: 2023-07-30

中图分类号： S668.4

基金资助:甘肃农业大学“伏羲杰出人才培育计划”(Gaufx-03J02)

通讯作者: * bhch@gsau.edu.cn

引用本文:

陆洋, 张志强, 卢世雄, 毛娟, 陈佰鸿. 草莓Trihelix转录因子家族的鉴定及表达分析[J]. 农业生物技术学报, 2024, 32(9): 2021-2032.
LU Yang, ZHANG Zhi-Qiang, LU Shi-Xiong, MAO Juan, CHEN Bai-Hong. Identification and Expression Analysis of Trihelix Transcription Factor Family in Strawberry (Fragaria vesca). 农业生物技术学报, 2024, 32(9): 2021-2032.

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https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.09.006 或 https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I9/2021

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