Isolation and Pathogenicity Analysis of Soilborne Pathogenic Fungi in the Rhizosphere of Pinellia ternata
WANG Chao-Yi1, WEI Xue-Jiao1, LI Kai-Yang2, LUO Qian1, ZHOU Dan1,*
1 School of Biological Science, Guizhou Education University, Guiyang 550018, China; 2 Hezhang County Agriculture and Rural Bureau of Bijie City, Hezhang 553200, China
Abstract：Continuous cropping barriers of Pinellia ternata have become an important factor limiting its yield and quality. To clarify the types of pathogenic fungi in the soil of multi-year continuous cropping of P. ternata,fungi were isolated and purified from the soil after 7 years of continuous cropping in this study. Pathogenic fungi were screened and identified via in vitro inoculation methods, and their pathogenicity was evaluated. To further understand the occurrence pattern of P. ternata root rot, the biological characteristics of the pathogenic fungi were investigated, including their growth conditions under different temperatures, light conditions, pH levels, carbon sources, and nitrogen sources were further investigated, along with the lethal temperature for the fungi. Experimental results showed that 22 fungal strains were isolated from the soil of 7 years of continuous cropping of P. ternata, among which 4 strains exhibited pathogenic ability: Fusarium falciforme E, F. solani N, F. keratoplasticum H, and F. oxysporum O. All the above pathogenic bacteria could utilize various carbon sources, grow rapidly at a temperature of 25~30 ℃, pH 9~10 and under light conditions, and lose their growth activity when treated at 56~61 ℃. This study provides an experimental basis for the identification and control of pathogenic fungi in the soil of continuous cropping of P. ternata.
王朝奕, 韦雪娇, 李开阳, 罗倩, 周丹. 半夏根际土壤病原真菌的分离及致病力分析[J]. 农业生物技术学报, 2025, 33(12): 2691-2702.
WANG Chao-Yi, WEI Xue-Jiao, LI Kai-Yang, LUO Qian, ZHOU Dan. Isolation and Pathogenicity Analysis of Soilborne Pathogenic Fungi in the Rhizosphere of Pinellia ternata. 农业生物技术学报, 2025, 33(12): 2691-2702.
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