Study on the Role of UeSho1 in Regulating the Pathogenicity of Ustilago esculenta
ZHANG Ya-Fen1,*, FENG Yu-Chen1,*, HU Ying-Li1, YE Zi-Hong1, TANG Jin-Tian1, WU Jun-Cheng1, XIE Lu2, HU Miao-Dan2,**
1 College of Life Sciences/Key Laboratory of Microbiological Metrology, Measurement & Bio-product Quality Security, State Administration for Market Regulation, China Jiliang University, Hangzhou 310018, China; 2 Shaoxing City Academy of Agricultural Sciences, Shaoxing 312000, China
Abstract：Ustilago esculenta exclusively infected Zizania latifolia and could enlarge the host stems to form edible Jiaobai. Its pathogenicity was closely related to the formation and phenotypic differentiation of Jiaobai. It was found that synthetic high osmolarity- sensitive protein (Sho1), as an environmental signal receptor upstream of the high osmolarity glycerol (HOG) pathway, plays an important role in the regulation of fungal pathogenicity. In order to study the role of Sho1 in the regulation of the pathogenicity of U. esculenta, the UeSho1 gene (GenBank No. MW768948.1) was cloned and identified. Its open reading frame was 1 137 bp in length with one 126 bp intron, encoding 336 amino acids including a SH3 conserved domain. It was obviously induced expressed during in vitro mycelial growth and infection process of U. esculenta. Further in vitro growth and infection experiments were carried out after knocking out UeSho1 in U. esculenta, and the results showed that the deletion of UeSho1 did not affect the haploid morphology, growth rate, or mating process of U. esculenta in vitro, but it inhibited the filamentous growth of mycelia, slowed down the infection process, and suppressed the expression of b signaling pathway genes related to pathogenicity. Additionally, the absence of UeSho1 did not affect the growth state of U. esculenta in vitro under osmotic, temperature or oxidative stress. This study reveals the critical role of UeSho1 in the regulation of U. esculenta pathogenicity and provides a theoretical basis for further exploration of its role in the interaction between U. esculenta and Zizania latifolia, particularly in inducing host stem swelling.
张雅芬, 冯雨宸, 胡映莉, 叶子弘, 汤近天, 吴骏成, 谢舻, 胡妙丹. UeSho1调节菰黑粉菌致病力的作用研究[J]. 农业生物技术学报, 2025, 33(5): 1117-1129.
ZHANG Ya-Fen, FENG Yu-Chen, HU Ying-Li, YE Zi-Hong, TANG Jin-Tian, WU Jun-Cheng, XIE Lu, HU Miao-Dan. Study on the Role of UeSho1 in Regulating the Pathogenicity of Ustilago esculenta. 农业生物技术学报, 2025, 33(5): 1117-1129.
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