Identification of the HSF Gene Family in Flowering Chinese Cabbage (Brassica rapa var. parachinensis) and Its Expression Analysis Under High Temperature Stress
JIANG Ding1, YUAN Fan-Chong1, SUN Feng-Lin2, LI Guang-Guang2, LEI Shi-Kang1, ZHENG Yan-Song2,*
1 Guangzhou Academy of Agricultural Sciences, Guangzhou 510335, China; 2 Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou 510335, China
Abstract：Heat shock transcription factor (HSF) plays an important role in regulating heat response and tolerance in plants. In order to further investigate the function of HSF genes in flowering Chinese cabbage (Brassica rapa var. parachinensis), this study used bioinformatics methods to systematically identify and analyze the HSF gene family and its molecular characteristics, and analyzed the gene expression patterns under high temperature stress. The results showed that a total of 39 HSF family genes were identified at the whole genome level of flowering Chinese cabbage, with protein sequence lengths ranging from 140 to 487 aa, molecular weights ranging from 16.10~53.82 kD, and theoretical isoelectric points ranging from 4.63 to 10.08. Protein subcellular localization prediction showed that 38 HSF proteins in flowering Chinese cabbage were located in the nucleus. The gene structure of most members of the HSF gene family in flowering Chinese cabbage was relatively conserved, with most genes contained 2 exons and 1 intron, as well as highly conserved DBD domain, conserved motif, and HSF domain. The 39 HSF genes in flowering Chinese cabbage were unevenly distributed on 10 chromosomes, with chromosome 3 had the most members. In synteny analysis, there were 32 genes in flowering Chinese cabbage that had a synteny relationship with Arabidopsis thaliana. The comparative analysis of the phylogenetic relationship between the HSF genes in flowering Chinese cabbage and A. thaliana indicated that these members of the HSF gene family could be divided into 3 categories: A, B, and C, with varying numbers of groups, and there were 9 pairs of orthologous genes and 2 pairs of paralogous genes. In addition, a large number of cis-acting elements related to plant hormones and stress response were found to be widely distributed in the promoter regions of HSF genes in flowering Chinese cabbage. qRT-PCR analysis showed that high temperature stress significantly induced the specific expression of most HSF genes in flowering Chinese cabbage (P<0.05), with 37 HSF genes showed up-regulation and 2 HSF genes showed down-regulation. Ten HSF genes highly expressed in the leaves or roots of flowering Chinese cabbage seedlings were screened, suggested that these genes play an important role in regulating the response of flowering Chinese cabbage to heat shock. This study provides a theoretical basis for understanding the characteristics of the HSF gene family in flowering Chinese cabbage and analyzing the functions of HSF family members in response to high temperature stress.
江定, 袁凡崇, 孙峰林, 李光光, 雷世康, 郑岩松. 菜心HSF基因家族鉴定及其在高温胁迫下的表达分析[J]. 农业生物技术学报, 2025, 33(5): 972-988.
JIANG Ding, YUAN Fan-Chong, SUN Feng-Lin, LI Guang-Guang, LEI Shi-Kang, ZHENG Yan-Song. Identification of the HSF Gene Family in Flowering Chinese Cabbage (Brassica rapa var. parachinensis) and Its Expression Analysis Under High Temperature Stress. 农业生物技术学报, 2025, 33(5): 972-988.
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