1 Department of Basic, Shanxi Agricultural University, Jinzhong 030800, China; 2 College of Resources and Environment, Shanxi Agricultural University, Jinzhong 030800, China
Abstract：The diversity of color in mung bean (Vigna radiate) seed coat is one of the important phenotypic characteristics. Flavonoids are the key substances affecting the color of mung bean seed coat, and they have important medicinal value. There are great differences in nutritional and economic value for mung beans with different seed coat color. 'Zhangjiakou yellow mung bean' (ZH), 'Jin mung bean 8' (JL8) and 'Ji black green 0506' (JH) were used as experimental materials. The phenotypic parameters of mung bean were determined by non-contact spectrophotometer. The metabolites of mung bean seeds were detected and analyzed by widely targeted metabolomics technology. The key differential metabolites were screened by K-means clustering analysis and the correlation analysis was done with phenotypic parameters. The molecular mechanism of the nutritional value and colour formation were explored for the mung beans with different seed coat color. The 537 metabolites, 218 differential metabolites and 15 common differential metabolites were detected in the 3 mung beans. ZH shows significant differences in the distribution and accumulation of secondary metabolites, especially flavonoids, compared to JL8 and JH. Metabolome data analysis showed that selphinidin 3-glucoside and quercetin 3-O-β-D-glucosyl-(1→2)-rhamnoside might be the substances that cause the black color of the seed coat of JH. The luteolin 7-rutinoside, vitexin 2''-glucoside, nudifloramide and i-inositol were the causes of green accumulation in mung bean seed coat by the analysis of K-means clustering and correlation analysis. This study provides essential data for further revealing the pigments and the difference of nutrients of mung beans with different seed coat colors at the molecular level.
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