Identification and Analysis of Stigma Exsertion Genes in Sterile Lines of Foxtail millet (Setarial italica)
WANG Juan-Fei1, MA Hui-Fang1, XUE Hong-Li1, ZHAO Xiong-Wei2, GUO Jie3, SHI Guan-Yan1*
1 Industrial Crop Institute/Hou Ji Laboratory in Shanxi Province, Shanxi Agricultural University, Taiyuan 030000, China; 2 College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China; 3 College of Agriculture, Shanxi Agricultural University, Taigu 030801, China
Abstract：Stigma exsertion of millet sterile lines is a key trait affecting the efficiency of hybrid seed production of foxtail millet (Setarial italica) hybrids. The longer stigma and more obvious exsertion, which is more valuable for the seed production yield of hybrid seeds. In this study, stigma exertion length was measured among sterile lines 'Jinfen line 21A', 'Jinfen line 92A', '8116A', 'EMS-1A' and 'Fen 33A', while 'Jinfen line 92A' was tested with longest stigma exsertion length (0.66 mm), 'Jinfen line 21A' had the shortest stigma exsertion (0.06 mm). A total of 12 comparable foxtail millet stigma exsertion genes were identified using homologous alignment analysis, encoding 461~1 272 amino acid residues with molecular weight ranging from 50.173 90~138.045 10 kD, isoelectric point ranging from 5.05 to 9.13, and instability coefficient ranging from 30.80 to 50.92. Phylogenetic tree analysis demonstrated that all protein could be divided into 4 groups, and members of the groups had similar motifs and conservative domains. The Seita.2G100300 was homologous to the rice stigma exsertion gene by phylogenetic analysis. Further analysis of Seita.2G100300 at the transcriptional level showed that the expression of the gene was significantly lower than the control at heading stage and significantly increased at flowering stage (P<0.05). Correlation analysis showed a significant positive correlation between the expression level of Seita.2G100300 with the stigma exsertion length in the sterile line 'Jinfen line 92A' and '8116A' (P<0.05). This study integrated the stigma exsertion length phenotype with the expression analysis of related genes, offering novel insights into the mechanism underlying stigma exsertion in male-sterile lines of foxtail millet.
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