WEI Jing-Jing1,2, MA Li-Li1,2, ZHANG Ning1,2*, SI Huai-Jun1,2,
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Plant dehydrins (dehydrins, DHN) are a class of specific proteins expressed when plants are subjected to adversities such as drought and salinity. To investigate the function of potato (Solanum tuberosum) StDHN-2 gene in plant drought tolerance, in this study, the CDS of StDHN-2 gene was cloned from potato cultivar 'Atlantic' plantlets, and the fusion protein expression vector was constructed by homologous recombination, the subcellular localization results of StDHN-2 protein showed that the protein might be located in the nucleus. Based on the bioinformatics analysis of StDHN-2 gene, it was found that the genome had a total length of 931 bp, including a coding region sequence of 474 bp, which encoded 157 amino acids; The molecular weight of StDHN-2 protein was 16 659.06 D, and the theoretical isoelectric point was 7.23. The analysis showed that StDHN-2 protein was a hydrophilic protein without transmembrane domain. The secondary structure of the StDHN-2 protein was mainly the random coil, and there was no β-turn structure. By constructing a phylogenetic tree, it was found that StDHN-2 protein had the closest evolutionary relationship with Solanum verrucosum; The cis-acting element analysis showed that the promoter region of StDHN-2 gene contained ABRE, DRE, P-box, LTR and other elements related to abiotic stress response; The expression patterns of StDHN-2 gene were analyzed under PEG, NaCl, and abscisic acid (ABA) stress treatments by using qRT-PCR analysis, it was found that the gene was up-regulated under all 3 kinds of stress treatments. The results of the study preliminarily indicated that the StDHN-2 gene could be a good candidate gene for drought tolerance in potato and involved in the biological process of drought tolerance in plants. This study provides basic information for analyzing the drought response mechanism of potato StDHN-2 gene.
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