Abstract：Tail sequence is a primer sequence which is not belong to maize genome and can be widely applied in maize SSR primers. A lot of common maize SSR primers can be used in fluorescence detection system just by labeling 5` end of the tail sequence with fluorescence, then high-through and low cost detection could be achieved at the same time. A method of designing tail sequence was discussed, including design principle, design process, universality and PCR conditions.
收稿日期: 2007-01-04
通讯作者:
赵久然
引用本文:
匡猛 王凤格 赵久然 邢国风. 玉米SSR引物尾巴序列的设计方法[J]. , 2007, 15(6): 0-.
Meng KUANG Jiu-Ran ZHAO . A Method of Designing Tail Sequence for Maize SSR Primers. , 2007, 15(6): 0-.
