Abstract：OPW02-1756, OPO11-964, OPY13-661, OPB11-520, OPW05-766, OPV03-1365 and OPJ16-759, seven RAPD markers linked to the resistant powdery mildew genes in Chinese wild Vitis were reclaimed, cloned, sequenced and analyzed. The results showed that the real length of seven RAPD markers was 1756bp, 964bp, 661bp, 520bp, 766bp, 1365bp and 759bp respectively. Marker OPW02-1756 shows 94% to 98% identity with 3 EST sequences from European grapes. OPO11-964 shows 93 % identity with 1 EST sequence from Cabernet Sauvignon, shows 90% and 87% identity respectively with 2 EST sequences from green grape berries library, shows 83% identity with 1 EST sequence from multiple pathogen-infected sweet orange. OPO11-964 sequence has a biggest open reading frame of 444bp encoding 147 amino acids, has partial identity with 21 protein sequences of unknown functions from other lives. OPY13-661 sequence has identity with 16 EST sequences from European grapes, and shows 89% identity with 2 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay. OPB11-520 shows 94% identity with 4 genomic sequences of unknown functions of Arabidopsis thaliana. OPW05-766 shows 50% identity with GAG-POL precursor of wine grape. OPV03-1365 shows 81% to 88% identity with 6 rice genomic sequences, 84% to 91% identity with 3 sequences from Arabidopsis thaliana. OPJ16-759 shows 83% to 100% identity with 14 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay, 94% to 100% identity with 3 EST sequences from water stressed berry of Vitis vinifera var. Cabsau.
收稿日期: 2007-10-29
通讯作者:
张剑侠
引用本文:
张剑侠 王跃进 周邦军 徐伟荣. 中国野生葡萄抗白粉病基因RAPD标记的克隆及序列分析[J]. , 2008, 16(3): 0-.
Zhang Jian-xia Wang Yue-jin Zhou Bang-jun Xu Wei-rong. Cloning and sequence analysis of RAPD markers linked to the resistant powdery mildew genes in Chinee wild Vitis. , 2008, 16(3): 0-.
