Abstract：Keratin relates to the output of cashmere wool and the quality of fluff fiber. The purpose of this study is to research the change rule of differentially expressed protein of hair follicle in Inner Mongolia cashmere goat(Capra cashmere). Two- dimensional gel electrophoresis(2-DE) was used to separate total protein of hair follicle in 12 months and protein map for hair follicle cycle was established. Differentially expressed protein spots were identified and the expression trends of partial protein spots were analyzed by mass spectrometry. The results indicated that 255 protein spots were distributed at pI 4~7 and their molecular weight were 30~85 kD in each 2-DE maps of 12 months. 20 differentially expressed protein spots were found with Image Master 2-D platinum software. Finally, 12 protein spots were successfully identified by mass spectrometry, and they were mainly keratins (K) which participated in cell biological process and played important biological function. The 2-DE maps of 12 months were divided as follows: Four months of November, December, January and February were classified as hair follicle catagen; March and April were classified as hair follicle telogen, when proteins expression significantlly reduced; six months of May, June, July, August, September and October were classified as flourishing period with the highest expression. Preliminary analysis results showed that the expression level of K1, K5, K71 and K25 were the highest in flourishing period, which might promote the growth of hair follicle. At the same time, the expression level of K83 and K10 were the highest in the period of telogen, which would have the relationship with hair follicle telogen. In conclusion, excellent protein expression profiles for hair follicle were established and differentially expressed protein spots were successfully analyzed in this study, which will provide basic data for research on hair follicle cycle based on proteomics for Inner Mongolia Cashmere goat and searching marker protein.
