Abstract：Barley stripe disease is caused by Pyrenophora graminea, and is a worldwide disease. To explore the interaction mechanism between Hordeum vulgare and Pyrenophora graminea, total protein of Ganpi 6 and Isotta leaves which were inoculated with Pyrenophora graminea (DWC) for 7 and 21 days were extracted to analyze proteomic changes. Twenty eight protein spots were found which showed a change of more than 1.4-fold between the inoculated and the mock-inoculated samples of Ganpi 6 and Isotta, of which 4 were up-regulated, 6 were down-regulated, 2 were induced and 2 were inhibited for Ganpi 6, and 3 were up-regulated, 4 were down-regulated, 4 were induced and 3 were inhibited for Isotta. The up-regulated proteins included ribulose-bisphosphate carboxylase activase A (No. 2 spot), actin (No. 9 spot), ribosome-recycling factor (RRF)(No. 10 spot), ATP synthase gamma chain(No. 11 and 27 spots), succinate dehydrogenase [ubiquinone] flavoprotein subunit (No. 15 spot) and predicted protein (No. 26 spot). The down-regulate protein spots included bas1 protein (No. 4 spot), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RuBisCo)(No. 3, 5, 12, 14, 16 and 18 spots), ATP synthase CF1 alpha subunit (No. 13 spot), Ycf3 (No. 17 spot) and alpha-1,4-glucan-protein synthase (UTPG)(No. 28 spot). The induced expression protein spots included predicted protein (No. 6 spot), lipoxygenase 2 (LOX2)(No. 7 spot), light harvesting chlorophyll a/b-binding protein (LHCP)(No. 19 spot), 3-phosphoglycerate kinase (PGK)(No. 20 spot), lectin (No. 21 spot) and ATP synthase gamma chain (No. 22 spot). The inhibited protein spots included RuBisCo (No. 1, 8, 24, 25 spots) and ribulose bisphosphate carboxylase small chain (RuBPCase)(No. 23 spot). These protein spots were identified by MALDI -TOF/ TOF MS and protein database searching by MASCOT program. The functions of the 28 protein were related to photosynthesis, protein synthesis, plant defense responses, cell signaling transduction, cellulose biosynthesis, and response to resist disease. These differentially expressed proteins might be related to the response to infection by Pyrenophora graminea. The results elucidate the proteomic basis of barley varieties with different resistance to Pyrenophora graminea.
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