Abstract：Long noncoding RNA (LncRNA) is involved in a variety of biological processes in mammals, and the current research on its role in hair follicle formation process is relatively small, therefore, studying the effect of LncRNA on hair follicle development has become a new direction to understand the biological process of hair follicle formation. In order to verify the RNA-Seq results of skin tissue in the non-period of the development of the Subo Merino sheep (Ovis aries) and the difference in the expression of predicted target genes, the expression of two LncRNA 00202353 and LncRNA 00316173 and their target genes in skin tissue, which were differentially expressed in skin tissue during the development of Subo Merino sheep and their target genes associated with hair follicle development were detected by qRT-PCR. The results showed that the expression level of LncRNA 00202353 and its target gene secreted frizzled-related protein 2 (SFRP2) at 85 day of embryonic stage was extremely significantly higher than 65 and 135 d of embryonic stage, 7 and 30 d after birth (P＜0.01). The expression level of LncRNA 00316173 and its target gene insulin-like growth factor 2 (IGF2) at 65 d of embryonic stage was extremely significantly higher than 135 d of embryonic stage, 7 and 30 d after birth (P＜0.01). Analysis of the hair follicle developmental stage of the Subo Merino sheep was divided into developmental and maturity stages, the expression levels of LncRNA 00202353 and its target gene SFRP2, LncRNA 00316173 and its target gene IGF2 were extremely significantly higher in the hair follicle development than in the hair follicle maturation (P＜0.01). It indicatd that LncRNA 00202353 and LncRNA 00316173 and their target genes were involved in the development of hair follicles. These resullts provide important reference value for further studies on the development of hair follicles in LncRNAs, and supply research clues for exploring the biological processes of the periodic changes of ultrafine woolen sacs.
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