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2025年8月1日 星期五
农业生物技术学报  2019, Vol. 27 Issue (1): 89-96    DOI: 10.3969/j.issn.1674-7968.2019.01.010
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
不同发育阶段小尾寒羊皮肤毛囊特异性miRNA的鉴定
何玉龙1, 2, 陶宇1, 李勇2, 杨易2, 周学章2, 吴月红1, 2, *
1 浙江理工大学 生命科学学院,杭州 310018;
2 宁夏大学 西部特色生物资源保护与利用教育部重点实验室,银川 750021
Identification of Specific miRNA in Skin Follicles of Small Tail Han Sheep (Ovis aries) at Different Developmental Stages
HE Yu-Long1, 2, TAO Yu1, LI Yong2, YANG Yi2, ZHOU Xue-Zhang2, WU Yue-Hong1, 2, *
1 College of Life Science, Zhejiang Science and Technology University, Hangzhou 310018, China;
2 Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China
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摘要 小尾寒羊(Ovis aries)是我国优良的肉裘兼用型绵羊品种,因其生长速度快、产羔率高而闻名。microRNA (miRNA)是参与转录后表达水平调节的重要因子,可参与调控多种动物的皮肤和毛囊的发育。为了阐明miRNAs在小尾寒羊皮肤毛囊发育过程中的调控作用,本研究利用高通量测序技术分析了成年(XWHY_1)、出生30 d (XWHY_2)、刚出生(XWHY_3)等3个时期小尾寒羊皮肤毛囊组织的miRNAs表达谱,在3个不同发育时期共鉴定出1 062个miRNAs,包括已知miRNAs 160个,新发现miRNAs 902个。对鉴定出的miRNAs进行统计分析,在XYHY_1、XYHY_2和XYHY_3中特异性表达的miRNAs数目分别为69、127和73个,而在3个时期共表达的miRNAs为666个。统计分析采用费舍尔精确检验(Fisher exact test)和卡方检验(Chi-square test),对同时满足|log2 (Fold_Change, FC)|≥1且P≤0.05的差异表达miRNAs进行统计,经XWHY_1、XWHY_2、XWHY_3两两比较筛选出339个差异表达miRNAs,去重复后得到226个差异表达miRNAs;进一步进行靶基因预测,得到2 645个靶基因。对差异表达miRNAs进行京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)通路(Pathway)富集,2 645个靶基因注释到181个信号通路;对差异表达miRNAs进行基因本体(gene ontology, GO)富集分析,主要包括转录调控、细胞膜组成和离子结合等过程。本研究为在miRNA水平解析皮毛/绒性状形成的分子机理提供了基础资料,同时为保持小尾寒羊现有优良品质的同时通过人工调控手段提高毛/绒产量及品质提供了新的思路。
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何玉龙
陶宇
李勇
杨易
周学章
吴月红
关键词 miRNA高通量测序皮肤毛囊小尾寒羊    
Abstract:Small Tail Han sheep (Ovis aries) is a sheep breed with both excellent meat and fur in China, and famous for its fast growing speed and high lambing rate. microRNA (miRNA) is post-transcriptional regulator factor which is involved in the regulation of development of skin and hair follicles in various animals. In the present study, in order to illustrate this regulation in Small Tail Han sheep, the miRNA expression profiles of the skin and hair follicles during adult (XWHY_1), postnatal 30 d (XWHY_2) and newborn (XWHY_3) were established and analyzed by using high throughput sequencing technology. The results showed that total 1 062 miRNAs, including 160 known miRNAs and 902 new miRNAs, were identified in these 3 developmental periods. By statistical analysis of these miRNAs, it was found that there were 69, 127, and 73 specifically expressed miRNAs in XYHY_1, XYHY_2 and XYHY_3, respectively. Total 666 miRNAs were commonly expressed in the 3 periods. Statistical analysis of differential expression of miRNAs was conducted by Fisher exact test and Chi-square test, and meet the condition of |log2(Fold_Change, FC)|≥1 and P≤0.05. By comparing XWHY_1, XWHY_2 and XWHY_3, 339 differentially expressed microRNAs were screened, 226 differentially expressed microRNAs were obtained after removing the repetition, and 2 645 target genes were predicted. Differentially expressed miRNAs were enriched by Kyoto encyclopedia of genes and genomes (KEGG) Pathway, results showed that 2 645 target genes were annotated to 181 signaling pathways. Differentially expressed of miRNAs was enriched by gene ontology (GO) and showed to be mainly participated in transcriptional regulation, formation of cell membrane and ion binding. The present study provides basic data to analyze the molecular mechanism of fur/velvet formation at miRNA level, and also provides a new thought to improve the yield and quality of wool/velvet of Small Tail Han sheep by means of manual regulation while maintaining its existing excellent quality.
Key wordsmiRNA    High-throughput sequencing    Skin    Hair follicle    Small Tailed Han sheep
收稿日期: 2018-05-07     
ZTFLH:  S826  
  Q38  
基金资助:国家自然科学基金(No. 31460585)和浙江理工大学启动基金(No. 16042062-Y)
通讯作者: *,wuyh_77@126.com   
引用本文:   
何玉龙, 陶宇, 李勇, 杨易, 周学章, 吴月红. 不同发育阶段小尾寒羊皮肤毛囊特异性miRNA的鉴定[J]. 农业生物技术学报, 2019, 27(1): 89-96.
HE Yu-Long, TAO Yu, LI Yong, YANG Yi, ZHOU Xue-Zhang, WU Yue-Hong. Identification of Specific miRNA in Skin Follicles of Small Tail Han Sheep (Ovis aries) at Different Developmental Stages. 农业生物技术学报, 2019, 27(1): 89-96.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.01.010     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I1/89
 
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