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2025年8月14日 星期四
农业生物技术学报  2019, Vol. 27 Issue (6): 1042-1050    DOI: 10.3969/j.issn.1674-7968.2019.06.010
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
越背花毛蒙古马皮肤组织中色素生成相关基因TYRTYRP1DCT的表达分析
赵若阳1, 李超1, 图格琴1, 陶克涛1, 拉希玛1, 王文兴2, 黄博光2, 白东义1,*, 芒来1,*
1 内蒙古农业大学 动物科学学院/内蒙古自治区马属动物遗传育种与繁殖重点实验室/农业农村部马属动物遗传育种与繁殖科学观测实验站/内蒙古农业大学 马属动物研究中心,呼和浩特 010018;
2 内蒙古中蕴马产业集团,锡林浩特 026000
Expression Analysis of Melanogenesis Related Genes TYR, TYRP1 and DCT in Tobiano Mongolian Horse (Equus caballus) Skin Tissue
ZHAO Ruo-Yang1, LI Chao1, BAO Tuge-Qin1, MONGKE Togtokh1, Laxima1, WANG Wen-Xing2, HUANG Bo-Guang2, BAI Dong-Yi1,*, MANG Lai1,*
1 College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics/Breeding and Reproduction/Equine Research Center, Scientific Observing and Experimental Station of Equine Genetics/Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China;
2 Inner Mongolia Zhong Yun Horse Industry Group, Xilinhot 026000, China
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摘要 哺乳动物毛色形成的分子机制一直以来是动物遗传育种领域研究的热点,自然界中多种多样的动物毛色引起了许多研究者的兴趣,其中马(Equus caballus)的毛色种类繁杂,是马品种登记中不可或缺的一项内容,马毛色分子机理的研究一直以来非常重要。目前的研究大多为群体间的研究,鲜少有报道从个体毛囊色素合成的角度研究马毛色。越背花毛蒙古马(Tobiano Mongolian Horse)毛色特别,形成机理更加复杂。色素合成过程涉及的基因数量众多,调控网络复杂。其中酪氨酸酶基因(tyrosinase,TYR)、酪氨酸相关蛋白酶1基因(tyrosinase-related protein 1, TYRP1)及酪氨酸酶相关蛋白2基因(tyrosinase-related protein 1, TYRP2)(现称多巴色素异构酶(dopachrome tautomerase, DCT)),是色素合成通路中重要的3个下游基因。本实验采用石蜡包埋组织切片苏木素-伊红(hematoxylin-eosin staining, HE staining)染色技术初步探究了越背花毛蒙古马毛囊结构及色素分布,qRT-PCR方法检测不同颜色部位TYRTYRP1DCT基因的表达差异,后续分别利用Western blot和免疫荧光技术对3个基因相应蛋白进行定量定位分析。结果显示,越背花毛蒙古马不同颜色(黑, 白)皮肤组织中色素分布明显不同,真皮层厚度不同,毛囊毛球直径有差异。3个基因的mRNA与蛋白表达量在不同颜色皮肤组织中的表达有明显差异,蛋白的表达基本定位于毛囊毛球的毛母质部位。最终得出结论,色素合成下游基因TYRTYRP1DCT的表达差异影响了越背花毛蒙古马不同颜色皮肤组织中的色素合成,对毛发色素沉积过程具一定的调控作用。本研究为深入研究越背花毛蒙古马毛色分子机理提供资料,并为其他哺乳动物毛色研究提供参考借鉴。
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作者相关文章
赵若阳
李超
图格琴
陶克涛
拉希玛
王文兴
黄博光
白东义
芒来
关键词 越背花毛蒙古马皮肤毛囊色素生成    
Abstract:Mammals coat color molecular mechanism has always been hotspot in animal genetics and breeding, variety of coat colors in nature arouse the interest of many researchers; horses (Equus caballus) with different complex colors, which is indispensable to the horse registration in breeding, and the molecular mechanisms of horse coat color is very important. Recent years, most researches are focused on breeds, few researches are from the perspective of the individuals and hair follicle pigment synthesis. Since Tobiano Mongolian Horse has unique coat color, the formation mechanism must be more complex. Pigment synthesis involves a large number of genes and complex regulatory networks. Tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (TYRP2) which is now called dopachrome tautomerase (DCT) are 3 major genes at downstream of melanogenesis pathway. Paraffin-embedded tissue sections were stained with hematoxylin-eosin (HE) to analyze hair follicle structure and pigment distribution. qRT-PCR was used to detect the mRNA expression levels of TYR, TYRP1 and DCT genes in different color skins. The results showed Western blot (WB) and immunofluorescence (IF) were used to quantitatively locate their corresponding proteins. The pigment distribution in different color (black and white) skin tissues and the length of hair follicle dermal papillae was significantly different, meanwhile, the thickness of dermis was different, and the diameter of hair follicle hairball was slightly different. The mRNA and protein expressions of 3 genes were significantly different in different color skin tissues, and the protein expression was basically located in the hair matrix of hair follicle dermal papillae. The results showed TYR, TYRP1 and DCT, downstream genes of melanogenesis, might affect the synthesis of pigment in the different color skin tissues of Tobiano Mongolian horses, and can be the candidate regulatory factors in the process of hair pigment deposition. It will present information for the in-depth study of the molecular mechanism of Tobiano Mongolian Horse coat color and provide reference for other mammals.
Key wordsTobiano phenotype Mongolian Horse    Skin    Hair follicle    Melanogenesis
收稿日期: 2019-02-03     
ZTFLH:  S821.2  
基金资助:国家自然科学基金(No. 31472070;No.31360538);国家重点研发计划项目(No. 2017YFE0108700);内蒙古自治区自然科学基金重大项目(No. 2017ZD06);内蒙古自治区科学技术厅项目(No. 2016030020);内蒙古农业大学科技成果转化专项资金动植物新品种选育(培育)项目(No. YZGC2017001)
通讯作者: dmanglai@163.com;baidongyi1983@163.com   
引用本文:   
赵若阳, 李超, 图格琴, 陶克涛, 拉希玛, 王文兴, 黄博光, 白东义, 芒来. 越背花毛蒙古马皮肤组织中色素生成相关基因TYRTYRP1DCT的表达分析[J]. 农业生物技术学报, 2019, 27(6): 1042-1050.
ZHAO Ruo-Yang, LI Chao, BAO Tuge-Qin, MONGKE Togtokh, Laxima, WANG Wen-Xing, HUANG Bo-Guang, BAI Dong-Yi, MANG Lai. Expression Analysis of Melanogenesis Related Genes TYR, TYRP1 and DCT in Tobiano Mongolian Horse (Equus caballus) Skin Tissue. 农业生物技术学报, 2019, 27(6): 1042-1050.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.06.010     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I6/1042
 
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