Abstract:SPF chickens infected with chicken reticuloendotheliosis virus(REV) induced immunosuppression, Levels of IFN-gamma(IFN-γ) in chicken spleen lymphocytes were measured by double mAb sandwich ELISA. The results showed that chickens grew tardily and appeared the typical symptom of immunosuppression such as severe atrophy in central immune organs. IFN-γ ELISA demonstrated significant increased IFN-γ production in spleen lymphocytes at 7 days post-infection (dpi) compared to the age-matched controls. Until 15 dpi, the IFN-γ levels was the highest and then decreased, but showed markedly higher than the controls. This is the first report of IFN-γ production in SPF chickens infected with REV measured by directly quantitative method.