Abstract：Abstract: β-1.4-N-acetylgalactosaminyl-transferase(Galnact2) is responsible for the first committed step in the synthesis of mucin-type O-glycans on protein substrates。In this study, primers flanking the galnact2 c.363 C>G mutation were designed according to the known chicken galnact-2 DNA sequence. This polymorphism was genotyped in 276 animals from six chicken breeds including White Recessive Rock, Xinghua, Taihe Silk, Baier Huang, Kangle, Nancheng Black, and in a F2 full sib resource population by crossing White Recessive Rock with Xinghua using PCR-XhoI-RFLP. The results showed that：(1) The genetic polymorphism of the Galnact2 c.363C>G mutation was abundant; (2) This polymorphism was significantly associated with body weight at all ages measured. Individuals with the GG genotype had heavier body weight (BW) at the ages of 1, 21, 28, 35, 49, 56, 63, 77 and 84 days than those with the CC genotype (p<0.01). GC individuals had heavier BW at the ages of 1, 21, 56, 63 days (p<0.01), and 35, 49, 77 and 84 days (p<0.05) than CC individuals. There was no significant difference between GC and GG animals for BW at all ages measured; (3) This polymorphism was not significantly associated with fatness traits including fat pad under the skin, abdominal fat weight, width of fat band, crude fat content in breast muscle and crude fat content in leg muscle.
收稿日期: 2007-04-20
通讯作者:
黄路生*
引用本文:
刘三凤 聂庆华 张细权 张志燕 任军 黄路生*. 鸡galnact2基因c.363C>G位点的多态性及其与体重、脂肪沉积性状的相关性[J]. , 2007, 15(6): 0-.
. Genetic Diversities of the Galnact2 c.363C>G Mutation and Its Association with Body Weight and Fat Deposition in Chicken. , 2007, 15(6): 0-.
