Abstract：Objective To explore the correlation between the polymorphism of Single nucleotide polymorphisms(SNPs) of Resistin and the reguration of fat diposition. Methods Screening formutations in the entire coding region for the Resisin gene were Performed by the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in Lantang pig、Luchuan pig、Lingao pig、Landrace、Large white and duroc pigs. Results Detected results of genotype demonstrate that no polymorphism in exon-1 and exon-3,two genotypes in exon-2(CC、GG),three genotypes in promoter region(CC、TT、CT),six genotypes in intron-1(EE、FF、GG、EF、EG、FG) and intron-2(LL、MM、NN、LM、LN、MN). Variance analysis revealed that Resistin gene had a high linkage with their growth and fatness traits.Conclusion There are SNPs in resistin gene in pigs ,and have association of these SNPs with fat diposition and obesity in pigs.
收稿日期: 2007-07-26
通讯作者:
高志杰
引用本文:
高志杰 刘铀. 六个品种猪胰岛素抵抗素基因单核苷酸多态性分析[J]. , 2008, 16(3): 0-.
. Analysis of RESISTIN Gene in Six Pig Breeds by PCR-SSCP. , 2008, 16(3): 0-.
