Abstract：Three pairs specificity primers was designed for amplification of adiponectin gene in geese according to the known sequence of chicken, duck , etc. The sequence of adiponectin gene in geese was obtained by PCR amplification and splicing. It contains two exons and one intron. The coding sequence is 738 bp and codes 245 amino acids. The coding sequence and the corresponding protein of geese adiponectin shares the highest homology with duck′s, which are 94.85% and 95.51% respectively, then was chicken’s, the lowest homology with mammals’. The predicted molecular weight (MW) and isoelectric point (pI) are 26603.3 Da and 5.19 respectively, which are highly similar to the above animals′. Reverse transcription-PCR revealed that geese adiponectin mRNA displayed high expression levels in skeletal muscle, fat tissue, heart and stomachus muscularis ,middle expression in small intestine, stomachus glandularis, kidney and lung, and weak expression in liver, spleen, ovaries and diencephalons. The result will be helpful for further studying the function and mechanism of the adiponectin gene in geese.
