Abstract：Spermine is one of the essential factors for cell proliferation and differentiation. The spermine in small intestine not only plays a role in regulating intestinal epithelial cell proliferation and apoptosis and promoting intestinal development, but also plays a role in regulating intestinal digestion and absorption. This experiment was conducted to study the effects of exogenous spermine on polyamine metabolism in female goose (Anser cygnoides) duodenum. Geese were fed spermine at 5 and 10 mg/kg (body weight). The expression of key genes related to polyamine metabolism and the content of polyamines in female geese duodenum were measured by qRT-PCR and high performance liquid chromatography. The gene expression levels of ornithine decarboxylase antizyme inhibitor (AZIN) 1, ornithine decarboxylase antizyme 1 (OAZ1), spermine synthase (SPMS), spermidine/spermine-N1-acetyltransferase (SSAT) and acetyl-polyamine oxidase (APAO) in the duodenum of female geese treated with spermine at 5 mg/kg were significantly higher than those in the control group (P<0.05), while the gene expression level of AZIN2 significantly decreased (P<0.05). The gene expression levels of AZIN1, OAZ1, ornithine decarboxylase (ODC), spermidine synthase (SPDS) and SSAT in the duodenum of female geese treated with spermine at 10 mg/kg were significantly higher than those in the control group (P<0.05). The contents of spermidine (65.08 vs 41.84 mg/kg) and spermine (255.79 vs 218.84 mg/kg) in the duodenum of female geese treated with spermine at 5 mg/kg were significantly higher than those in the control group (P<0.05). Spermidine content (56.20 vs 41.84 mg/kg) in the duodenum of female geese treated with spermine at 10 mg/kg was significantly higher than that in the control group (P<0.05), while spermine content (188.34 vs 218.84 mg/kg) significantly decreased (P<0.05). In summary, exogenous spermine affects the contents of spermidine and spermine in female goose duodenum through regulating the expression of key genes related to polyamine metabolism, while it had no significant effect on putrescine content. This study revealed the effects of exogenous spermine on the expression of key genes of polyamine metabolism and polyamine content in duodenum of female geese, which provides basic data for the study of spermine regulation on digestion and absorption of poultry, and provides a new way for the development of new functional feedstuffs.
杜菲, 易治鑫, 于海莹, 张燕, 李慧敏, 杨莉, 王钰曾, 姜冬梅, 康波. 精胺对雌鹅十二指肠多胺代谢关键基因表达及多胺含量的影响[J]. 农业生物技术学报, 2019, 27(8): 1445-1451.
DU Fei, YI Zhi-Xin, YU Hai-Ying, ZHANG Yan, LI Hui-Min, YANG Li, WANG Yu-Zeng, JIANG Dong-Mei, KANG Bo. Effect of Spermine on Expression of Key Genes Related to Polyamine Metabolism and Polyamine Contents in the Duodenum of Female Geese (Anser cygnoides). 农业生物技术学报, 2019, 27(8): 1445-1451.
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