Analysis on the Technical Key Points of Porcine (Sus scrofa) Somatic Cell Cloning Embryo Transfer
WU Ya-Lin1,2, LI Ji-Liang3, ZHOU Wei-Liang3, DONG Shu-Ren3, FENG Bao-Liang3, LIU Zhi-Guo2,*, MU Yu-Lian2, LI Kui2
1 Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528000, China; 2 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3 Tianjin Ningheyuan Swinebreeding Farm, Tianjin 301504, China
Abstract：As a necessary technique in the obtaining of transgenic and gene editing pigs (Sus scrofa), embryo transfer technology has an important influence on the cloning efficiency. In this paper, the key points of pig cloned embryos transfer, such as the selection of recipient sows, the concurrent nature of receptor sow estrus and transplanted embryos, the operation method, the anesthesia method, the time of transfer, the number of transplanted embryos and the post-operative nursing, are reviewed and discussed. This paper provides reference for the operation of pig somatic cell cloning embryo transfer.
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