VRTN及NR6A1基因多态性与苏尼特羊胸椎数变异的相关性及组织表达分析

doi:10.3969/j.issn.1674-7968.2019.05.010

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摘要胸椎数性状是生产中一类重要的经济性状，在绵羊(Ovis aries)上已确定的候选基因很少。本研究旨在研究脊椎发育相关基因(vertnin， VRTN)及核受体辅抑制子1基因(nuclear receptor subfamily 6 group A member 1, NR6A1)多态性与苏尼特羊胸椎数变异之间的联系及两个基因的组织表达水平。研究利用PCR研究了苏尼特羊VRTN和NR6A1基因的多态性及两个基因的SNP位点与多胸椎数性状的相关性，利用qRT-PCR技术检测了VRTN和NR6A1基因在不同胸椎数(胸椎数14根为T14组；胸椎数13根为T13组)苏尼特羊部分组织中的表达。结果表明，VRTN基因g.82533904C>T位点、NR6A1基因g.11206763T>C位点和g.11210075T>C位点与苏尼特羊胸椎数之间无显著关联(P＞0.05)，VRTN基因g.82533661C>A位点与苏尼特羊胸椎数之间存在极显著关联(P＜0.01)。NR6A1基因在不同胸椎数(T14组；T13组)苏尼特羊的心、肝、脾、肺、肾、肌肉和脂肪组织中均有表达，NR6A1基因在2种不同胸椎数苏尼特羊的心脏、肺脏、肾脏、肌肉和脂肪组织中的表达差异不显著(P＞0.05)，在脾脏组织中T13组与T14组之间表达差异处于显著水平(P＜0.05)，在肝脏组织中T13组与T14组之间表达差异处于极显著水平(P＜0.01)；VRTN基因在苏尼特羊的心、肝、脾、肺、肾、肌肉和脂肪组织中均有表达，在T14组中表达量高于T13组，T14组肌肉中表达量显著高于T13组(P＜0.05)，其他组织在不同胸椎数苏尼特羊之间的表达差异不显著(P＞0.05)。NR6A1基因在两种不同胸椎数苏尼特羊的组织中表达没有明显的规律，以上实验结果表明，绵羊VRTN基因g.82533661C>A位点对于选育多胸椎的苏尼特羊群体有一定指导意义，同时，NR6A1基因与苏尼特羊胸椎数性状之间没有明显关系，而VRTN基因可能是影响苏尼特羊胸椎数的重要基因之一。确定绵羊胸椎数候选基因有利于选育多胸椎数绵羊群体，能够有效提高肉羊产业的经济效益。

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	孙庆
	刘树军
	狄冉
	胡文萍
	王翔宇
	马琳
	张效生
	张金龙
	刘秋月
	储明星

关键词 ： VRTN基因, NR6A1基因, 胸椎数, 多态性, 组织表达, 苏尼特羊

Abstract：The number of thoracic vertebrae is an important economic trait in production, but few candidate genes have been identified in sheep (Ovis aries). The aim of this study is to explore the association between polymorphism of VRTN (vertnin) and NR6A1 (nuclear receptor subfamily 6 group A member 1) genes and thoracic vertebral number, and tissue expression levels of 2 genes were also detected. The association analysis of VRTN and NR6A1 polymorphisms with thoracic vertebral number were performed by PCR, and expression levels of VRTN and NR6A1 genes in Sunite sheep with different thoracic vertebral numbers (14 thoracic vertebral number, T14; 13 thoracic vertebral number, T13) were tested by qRT-PCR. The results showed that there were no significant association between g.82533904C>T, g.11206763T>C and g.11210075T>C and the thoracic vertebral number in Sunite sheep (P＞0.05), while there was a highly significant association between g.82533661C>A and the thoracic vertebral number in Sunite sheep (P＜0.01). NR6A1 expressed in heart, liver, spleen, lung, kidney, muscle and fat of Sunite sheep. There were no significant difference of expression levels between sheep with T14 and T13 in heart, lung, kidney, muscle and fat (P＞0.05), while there were significant difference in spleen (P＜0.05) and extremely significant difference in liver (P＜0.01) of Sunite sheep with different thoracic vertebral number. The expression levels of VRTN were all higher in Sunite sheep with T14 than those of T13 in heart, liver, spleen, lung and kidney, the difference of muscle was significant (P＜0.05), the difference of the other tissues were not significant (P＞0.05). There was no uniform expression pattern of Sunite sheep with different thoracic vertebral number. In summary, g.82533661C>A had potential application value for the selection of the Sunite sheep population with more thoracic vertebral number. At the same time, there was no significant association between NR6A1 and the thoracic vertebral trait of the Sunite sheep, and VRTN may be one of the candidate genes affecting the thoracic vertebral numbers in Sunite sheep. Determining candidate genes for thoracic vertebrae number trait in sheep is conducive to the selection of multiple thoracic vertebrae sheep population, which can effectively improve the economic benefits of the sheep industry.

Key words： VRTN NR6A1 Thoracic vertebral number Polymorphism Tissue expression Sunite sheep

ZTFLH:

S82

基金资助:中央级公益性科研院所基本科研业务费专项(No.2017ywf-zd-13)、国家肉羊产业技术体系专项(No.CARS-38)、中国农业科学院科技创新工程(No.ASTIP-IAS13)、农业科研杰出人才及其创新团队项目(No.农办人[2015]62号)和国家万人计划科技创新领军人才项目(No.W02020274)

引用本文:

孙庆,刘树军,狄冉,胡文萍,王翔宇,马琳,张效生,张金龙,刘秋月,储明星. VRTN及NR6A1基因多态性与苏尼特羊胸椎数变异的相关性及组织表达分析[J]. 农业生物技术学报, 2019, 27(5): 864-874.
SUN Qing,LIU Shu-Jun,DI Ran,HU Wen-Ping,WANG Xiang-Yu,MA Lin,ZHANG Xiao-Sheng,ZHANG Jin-Long,LIU Qiu-Yue,CHU Ming-Xing. Association Between Polymorphism of VRTN, NR6A1 Genes and Thoracic Vertebral Number Variation and Analysis of Their Tissue Expression in Sunite Sheep (Ovis aries). 农业生物技术学报, 2019, 27(5): 864-874.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.05.010 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I5/864

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