Expression of RXRs Genes in Sheep (Ovis aries) Reproductive Axis Related Organizations and the Relationship Between RXRA Gene Polymorphism and Seasonal Estrus
LI Hua-Zhen1,2,*, DI Ran2,*, Guo Xiao-Fei3, ZHANG Jin-Long3, ZHANG Xiao-Sheng3, MA Lin2, CHU Ming-Xing2,**, LIU Wu-Jun1,**
1 College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China; 2 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3 Tianjin Institute of Animal Sciences, Tianjin 300381, China
Abstract：Most of the sheep (Ovis aries) are seasonal estrus animal, which severely restricts the reproductive efficiency of sheep, and retinoid X receptors (RXRs) have received attention as seasonal estrus candidate genes. In order to investigate the differences in the expression of seasonal estrous candidate genes retinoid X receptors (RXRA, RXRB and RXRG) in sheep under different reproductive conditions, qRT-PCR was used to detect these 3 genes for the relative expression of 10 tissues about fallopian tube, brain, cerebellum, hypothalamus, pineal body, pituitary, ovary, fallopian tube, uterus, kidney and adrenal gland, in the follicular and luteal phases of Small Tail Han sheep (perennial estrus) ewes and the long day and short day conditions of the Sunite sheep (seasonal estrus) ewes. The Sequenom MassARRAY® SNP technique was used to detect the 2 SNPs of RXRA gene in perennial estrus sheep breeds (Small Tail Han sheep, Hu sheep and Cele black sheep) and seasonal estrus sheep breeds (Tan sheep, Sunite sheep and Tibetan sheep). The results showed that RXRA, RXRB and RXRG genes were widely expressed in the tissues of the 2 sheep breeds. The expression of RXRA gene in the pineal body of Sunite sheep in short day was significantly higher than that in long day (P<0.05), and the expression of follicular phase in the ovary of Small Tail Han sheep was extremely significant higher than the luteal phase (P<0.01), but the expression of follicular phase in the uterus was extremely significant lower than that in the luteal phase (P<0.01). The expression of RXRB gene in the pituitary of Sunite sheep in short day was significantly higher than in long day (P<0.05). The expression of follicular phase was extremely significant higher than that of luteal phase in the ovary of Small Tail Han sheep (P<0.01). RXRG gene was highly expressed in the hypothalamus, pituitary and pineal body of Sunite, and the expression level in pineal body in long day was extremely significant higher than in short day (P<0.01). The expression level in the uterus of the Small Tail Han sheep was extremely significant lower in the follicular phase than in the luteal phase (P<0.01). From genotyping by MassARRAY time-of-flight mass spectrometry, the study found the genotype frequencies and allele frequencies of the RXRA gene g.1699247 G>A and g. 1699276 G>A were distributed in the Small Tail Han sheep in accordance with the Hardy-Weinberg equilibrium, and were significantly different between seasonal and perennial estrus sheep (P>0.05). The above results implied that RXRs genes expression might be related to reproduction of sheep. These genes might be involved in the regulation of upstream genes of sheep seasonal estrus. The results of this study helps to understand the regulatory role of RXRs in seasonal estrus and estrus cycle transition.
李华振, 狄冉, 郭晓飞, 张金龙, 张效生, 马琳, 储明星, 刘武军. 绵羊繁殖轴相关组织RXRs基因的表达及RXRA基因多态性与季节性发情之间的关系[J]. 农业生物技术学报, 2019, 27(8): 1401-1409.
LI Hua-Zhen, DI Ran, Guo Xiao-Fei, ZHANG Jin-Long, ZHANG Xiao-Sheng, MA Lin, CHU Ming-Xing, LIU Wu-Jun. Expression of RXRs Genes in Sheep (Ovis aries) Reproductive Axis Related Organizations and the Relationship Between RXRA Gene Polymorphism and Seasonal Estrus. 农业生物技术学报, 2019, 27(8): 1401-1409.
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