Abstract：AtNHX1 antiporter gene from Arabidopsis was successfully introduced into white clover plants through their cotyledons, mediated by Agrobacterium tumefaciens. Transgenic plants resistant to Kanamycin were obtained after selection and regeneration. All of the transgenic plants were tested with PCR, Southern blot and Northern analysis, which indicated that AtNHX1 gene has already been integrated into white clover genome and expressed. Indoor salinity test showed that transgenic plants expressing AtNHX1 gene had significantly higher total leaf area and aboveground dry weight compared with non-transgenic controls, illuminating that vacuolar AtNHX1 gene can help to increase salt tolerance in white clover.
