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2025年4月3日 星期四
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高邮鸭和金定鸭发育早期骨骼肌发育及胰岛素样生长因子1受体基因(IGF-IR)表达分析
朱春红1,徐文娟2,胡艳1,朱文奇3,宋迟2,陈文峰2,李慧芳 3
1. 江苏省家禽科学研究所
2.
3. 中国农业科学院家禽研究所
Early Development and Developmental Expression of Insulin-like Growth Factor 1 Receptor(IGF-IR) mRNA in Gaoyou Duck and Jinding Duck(Anas platyrhynchos domestica) Skeletal Muscle
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摘要 类胰岛素生长因子(Insulin-like growth factors, IGFs)在动物生长发育及营养代谢中具有重要作用。为研究IGF-IR基因对鸭骨骼肌发育的影响,本实验采用实时荧光定量PCR方法研究了生长速度差异较大的高邮鸭和金定鸭发育早期(13、17、21、25、27胚龄和出雏后7日龄)骨骼肌(胸肌和腿肌)中IGF-IR基因表达差异,并分析其表达量变化与胸、腿肌发育的相关性。结果显示,IGF-IR mRNA在两个品种的胸肌和腿肌中均有表达,种间同一组织的表达变化规律一致,而不同组织中的表达模式不同,在胸肌中呈“波浪形”,在13胚龄时表达量最高,17胚龄表达量下降后,在21胚龄时表达量又上升,随后下降并维持在相对较低的水平,出雏后表达量略有上升;在腿肌中的表达趋势呈近似“L”形,在13胚龄时表达量较高,随后迅速下降并维持在相对较低的水平;品种内不同肌肉组织中IGF-IR mRNA表达变化显示在2个品种中除13胚龄外,胸肌中的表达量均极显著高于其腿肌(P<0.01)。二元变量相关性分析结果表明,不同品种中胸肌、腿肌IGF-IR mRNA的表达与胸肌、腿肌发育呈负相关,其中腿肌的相关性高于胸肌。结论:肌肉中IGF-IR基因表达具有显著的时空差异,骨骼肌中IGF-IR基因表达对鸭早期生长发育有负调控作用。对鸭发育早期骨骼肌中IGF-IR基因表达分析将为研究鸭早期发育激素调控机理提供基础资料。
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朱春红
徐文娟
胡艳
朱文奇
宋迟
陈文峰
李慧芳
关键词 IGF-IR基因胚胎期实时荧光定量PCR差异表达    
Abstract:Insulin-like growth factors play an important role in animal growth and nutrition metabolism. In order to research the effects of IGF-1 receptor(IGF-IR) gene on the skeletal muscle in early development stage of duck, the IGF-IR mRNA expressions on the skeletal muscle during Gaoyou duck and Jinding duck (Anas platyrhynchos domestica) embryonic stage on day 13, 17, 21, 25, 27 and 1 week after patch were detected using quantitative RT-PCR method in this experiment. The result indicated that the profiles of IGF-IR mRNA in muscle in two duck breeds were relatively consistent during early development which showed "wave" trend in pectorales and "L" trend in legs. Expression levels of IGF-IR gene on pectorals at 13 and 21 embryo age were higher, and the subsequent expression levels were somewhat lower at 25 and 27 embryo age and its expression levels in the newborn period(7 days) were slowly upward; the expression of duck IGF-IR gene on legs showed that the highest level appeared at 13 embryo age, and subsequently expression levels decreased to maintain a relatively low expression levels. Within the same breed, the expression levels of IGF-IR mRNA in pectorals were significantly higher than that in legs except for 13 embryo age(P<0.01). The result of bivariate correlate analysis showed that the expression of IGF-IR gene and weight development in pectorales and legs were negatively correlated and the correlate coefficient in legs was stronger. These results indicated that IGF-IR gene involved in the skeletal development, and different expression patterns in the pectorals and legs suggested IGF-IR gene may be related to the chest and leg muscle development and differentiation, and the expression of IGF-IR gene in muscle was important in the early growth and development in ducks. The analysis of IGF-IR gene in early development of skeletal muscle in Gaoyou duck and Jinding duck will provide theoretical basis for studing hormone regulation mechanism in duck.
Key wordsDuck    IGF-IR gene    Embryonic period    Quantitative real time PCR    Differential expression
收稿日期: 2012-07-31     
通讯作者: 李慧芳    
引用本文:   
朱春红1,徐文娟2,胡艳1,朱文奇3,宋迟2,陈文峰2,李慧芳 3. 高邮鸭和金定鸭发育早期骨骼肌发育及胰岛素样生长因子1受体基因(IGF-IR)表达分析[J]. , 2013, 21(2): 192-198.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2013/V21/I2/192
 
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